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EFFECT OF ANTIOXIDANT AND ITS CONCENTRATION ON THE SPERM QUALITY OF WALKING CATFISH Clarias batrachus POST CRYOPRESERVATION | ||
| Mesopotamia Journal of Agriculture | ||
| Article 12, Volume 53, Issue 4, December 2025, Pages 165-178 PDF (384.54 K) | ||
| Document Type: Research | ||
| DOI: 10.33899/mja.2025.163143.1676 | ||
| Authors | ||
| Muchlisin Zainal Abidin* 1; Luvi Syafrida Handayani2; Sri Riska Rahayu2; Nanda Muhammad Razi2; Mehmet Kocabas3; Filiz Kutluyer Kocabas4; Changxu Tian5; Hafizuddin Hafizuddin6; Nur Fadli7 | ||
| 1Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia | ||
| 2Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia | ||
| 3Department of Wildlife Ecology and Management, Karadeniz Technical University, Turkey | ||
| 4Faculty of Fisheries, Munzur University, Turkey | ||
| 5College of Fisheries, Guangdong Ocean University, China | ||
| 6Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia | ||
| 7Department of Aquaculture, Faculty of Marine and Fisheries, Universita Syiah Kuala, Banda Aceh 23111, Indonesia. | ||
| Abstract | ||
| The walking catfish Clarias batrachus is popular and affordable in Southeast Asian countries. The development of breeding technology for this species is ongoing, with sperm cryopreservation being a key aspect of study that supports its breeding program. Cryopreservation is an essential method in fish breeding, particularly for sustaining breeding initiatives that supply larvae throughout the years. However, this process can lead to oxidative stress, which may decrease sperm quality due to structural and physiological damage, including DNA fragmentation. Adding antioxidant to freezing medium could facilitate to mitigate these negative effects and maintain sperm quality. This study aims to evaluate the effectiveness of various types and concentrations of antioxidant on the quality of C. batrachus sperm post-cryopreservation. Four types of antioxidants were tested, namely Ascorbic acid, Myoinositol, Alpha tocopherol, and Butylated Hydroxytoluene, each at concentrations of 0, 20, 40, and 60 mg/L. Ringer solution, 5% DMSO and 5% egg yolk were used as extender and cryoprotectants, respectively. Sperm samples were stored in liquid nitrogen for 2 weeks, after which sperm quality was analyzed. Both the type and concentration of antioxidants significantly affected all measured sperm quality parameters (p<0.05). Ascorbic acid at 40 mg/L yielded the highest motility (62.33%), viability (66.00%), and fertility (61.67%) values. Electrophoresis analysis revealed no DNA fragmentation in any treatment, indicating that the genetic structure of the sperm was preserved. Ascorbic acid at 40 mg/L is the most effective antioxidant for cryopreservation of sperm C. batrachus. | ||
| Keywords | ||
| Local catfish; Myoinositol; Ascorbic acid; Sperm motility; DNA integrity | ||
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