Genetic Impact of Bottled Water Exposed to Different Temperatures on Drosophila melanogaster

Al-Amidi, Lamyaa Majeed; Hadi, Forat

doi:10.29079/qjvms.2025.159834.1084

	Genetic Impact of Bottled Water Exposed to Different Temperatures on Drosophila melanogaster
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Issue 2, December 2025, Pages 139-144 PDF (510.08 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2025.159834.1084
Authors
Lamyaa Majeed Al-Amidi^* ¹; Forat Hadi²
¹Babylon Education Directorate, Ministry of Education, Iraq
²Department of Biology, College of Education, University of Al-Qadisiyah, Iraq
Abstract
This study was conducted to explore the genetic effects of bottled water stored at different temperature conditions (25°C, 30°C, 40°C, and 50°C) on Drosophila melanogaster. The water samples were first analyzed chemically for lead, cadmium, and ethylene concentrations, followed by their incorporation into the insect's culture medium. The experiment examined phenotypic mutations, specifically vestigial wing and ebony body color traits, as well as changes in the mitotic index of third instar larvae. Thus, exposure to bottled water stored at 50°C induced a higher incidence of wing deformities (25%) and ebony body mutations than that of the control group (25°C; no abnormalities) as shown. In addition, there was a clear increase in mitotic activity, from 65 in the control to 76.3 at 50°C, these results pointing to genotoxic effects induced possibly by migration of toxic plastic-derived compounds into the water. The findings of this study highlight the need for rigorous storage of bottled water, and suggest the need for stronger regulation and measurement at the molecular level of migration of chemicals from packaging materials.
Keywords
bottled water; Drosophila melanogaster; temperature stress; genetic mutation; mitotic index

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