The rate and identification of isolates of Toxocara canis in Iraqi stray dogs.

Albayati, Hadeel Hadi; Jarad, Noor Idan; Mohammed, Nuha Qasim; Alhasanawi, Baneen Najm

doi:10.29079/qjvms.2025.164969.1115

	The rate and identification of isolates of Toxocara canis in Iraqi stray dogs.
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Special Issue ( 3 ) The 1st National and International Scientific Conference November 26 / 2025, January 2026, Pages 35-39 PDF (657.76 K)
Document Type: Al-Qadisiyah Conference 2025
DOI: 10.29079/qjvms.2025.164969.1115
Authors
Hadeel hadi Albayati^* ¹; Noor Idan Jarad²; Nuha Qasim Mohammed³; Baneen Najm Alhasanawi⁴
¹College of Veterinary Medicine University of Al-Qadisiyah Iraq
²Department of Microbiology, College of Veterinary Medicine, Al-Qadisiyah University, Iraq
³Zoonotic disease research unit, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
⁴College of Veterinary Medicine, University of Al-Qadisiyah, Iraq
Abstract
Using molecular and microscopic techniques, we assessed the prevalence of Toxocara canis in 270 stray dogs in Southern Iraq between January and June 2025. Season, age, and gender effects on prevalence were noted. Using the fecal flotation approach, we discovered that almost 23% of the men and women were infected, with 23.7% and 22.8% of the cases being identical. In February, when the temperature was about 26°C and the humidity was about 30%, the prevalence reached its highest point at 40%. Compared to adults (15.8%), the incidence was greater (P < 0.05) in younger dogs (34.9%). According to PCR, the identity of T. canis 3 and 4 was 99%, while the prevalence was 58% for 10 isolates that were identical to those obtained in Iran (accession no. KF577855.1). 99% similarity between the 10 isolates' Internal Transcribed Spacer (ITS1) regions and reference sequences of T. canis from China (accession no. JF837169.1) was found in the results.
Keywords
Identification; Toxocara canis; Dogs; PCR; Iraq

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