MicroRNA 153 as epigenetic biomarker for diagnosis of idiopathic pulmonary fibrosis

Yassin, Asseel; Al-Husseini, Alaa Mohammad Hasson

doi:10.29079/qjvms.2025.166513.1134

	MicroRNA 153 as epigenetic biomarker for diagnosis of idiopathic pulmonary fibrosis
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Special Issue ( 3 ) The 1st National and International Scientific Conference November 26 / 2025, January 2026, Pages 110-118 PDF (631.33 K)
Document Type: Al-Qadisiyah Conference 2025
DOI: 10.29079/qjvms.2025.166513.1134
Authors
Asseel Yassin^* ¹; Alaa Mohammad Hasson Al-Husseini²
¹Department of Anatomy and Histology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq
²Department Of Biology, College of Science, University of Al-Qadisiyah, Iraq.
Abstract
لإhe current study investigated the diagnostic relevance and expression profiles of free circulating miR-153 in newly diagnosed IPF and matched controls. So, these analyses have been evaluated as a total of 120 subjects, with 60 being the IPF and 60 being the age and gender matched healthy control subjects. Using the TRIzol reagent, collected blood samples were used in and extracted from them. In stem-loop RT-qPCR, quantification of the miR-153 and GAPDH (alternative gene) molecules is made. The analysis of the results concluded that there is an expression of the calculated values from the 2^−ΔCt expression analysis, while a performative diagnostic value is shown with the receiver operating characteristic analysis. The results now inform us that there tends to be a lower emission of miR-153 factors for IPF on the basis of comparison to the controls. In IPF, the mean droplet value for miR-153 was 28.63, while for the controls it was 25.34. The means thus stand to show that there was a decrease the expression of miR-153 in IPF when compared to the control subjects. In IPF the mean overall expression level was 0.25 ± 0.06 while the expression level in controls was 2.66 ± 0.91 (p < 0.001). IPF vs control diagnoses demonstrated excellent metrics IPF AUC 1.000, Sensitivity 100%, Specificity 100%. The significant downregulation of circulating miR-153 in IPF along with its exceptional diagnostic performance underscores its promise as a non-invasive biomarker for early detection and monitoring of the disease in idiopathic pulmonary fibrosis.
Keywords
biomarker; idiopathic pulmonary fibrosis; microRNA-153; RT-qPCR; serum expression

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