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Genetic diversity and molecular characterization of olive cultivars (Olea europaea L.) in Iraq using ISSR markers | ||
| Journal of University of Anbar for Pure Science | ||
| Volume 20, Issue 1, April 2026, Pages 110-118 PDF (2.07 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2025.162802.1527 | ||
| Authors | ||
| Anmar Kamil Alalwani* 1; Idrees AL-Jaf2; Mohammed Ahmed2; Ahmad kamis3; Basim Abed2 | ||
| 1Department of Biotechnology, College of Science, University of Anbar | ||
| 2Department of Horticulture and Landscape Architecture, College of Agriculture, University of Anbar | ||
| 3Department of Biology, College of Science, University of Anbar | ||
| Abstract | ||
| This study used ten different Olea europaea L. genotypes, including Ashrasi, Bashiqa, Manzanilla, Dakel, Al-Khudairi, Darmalali, Sorani, Qaisi, Nabali, and Ascolano. The genotypes were selected from seven sites representing the major areas of olive production and cultivation in Iraq. Genetic relationships among the 10 examined olive cultivars were determined using ISSR analysis, which revealed that 10 primers were highly efficient, generating 98 bands, 64 of which were polymorphic, yielding a mean polymorphism rate of 64.7%. These findings reveal significant genetic differentiation among the olive cultivars under study. Genetic similarity coefficients ranged from 0.11 to 0.83, indicating a broad spectrum of diversity. The Ashrasi cultivar was the most genetically distinct, suggesting it may be useful for enriching genetic diversity. In contrast, high genetic similarity was observed between Qaisi and Dakel, and between Darmalali and Al-Khudairi. These results were also supported by the dendrogram, which separated the cultivars into two major groups: one with higher similarity and the other with greater evolutionary distance, with Ascolano forming a separate branch, indicating its unique genetic identity | ||
| Keywords | ||
| Olive; ISSR markers; Molecular primers; Genetic similarity; Phylogenetic tree | ||
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