The effect of using Azotobacter spp and banana peels on the essential oil content of three basil varieties

ALSULTAN, Batool Abd

	The effect of using Azotobacter spp and banana peels on the essential oil content of three basil varieties
Al-Zahraa Journal for Health and Medical Sciences
Volume 4, Issue 1, March 2026, Pages 310-321 PDF (475.07 K)
Document Type: Research Article
Author
Batool Abd ALSULTAN^*
Department of Biology, College of Education for Pure Sciences ,University of kerbala, Iraq
Abstract
The current study, which was conducted in 2024 at the city of Alhindia in Kerbala, Iraq, sought to ascertain the effects of the bio fertilizer Azotobacter on the active parameters limonene, linalool, and pinene of three sweet basil plants (Ocimum basilicum L., Ocimum basilicum var. purple, and Ocimum basilicum var. citriodorum). With three replications and a factorial arrangement, the aforementioned experiment was set up using a Randomized Complete Block Design (RCBD). Two components were included in the study: banana peels (0, 100, 150, and 200 gm L⁻¹) and Azotobacter (0, 25, 50, and 100 g bacteria/m²).Extract basil fin powder using a Soxhlet apparatus. Measure active compounds by HPLC examination.The results indicate an increase in the volatile oil content of the effective compounds Limoninel , Linalool , Pinen when treated with banana peels at concentration 200 gmL-1(12.78,23.28 ,0.215 mg g-1)for O. basilicum L., ( 11.20,23.28,0.2025mg g-1) for O. basilicum var. Purple and ( 9.97 ,21.76,0.1925 mg g-1) for O. basilicum var. Citriodorum. The study suggests choosing the variety O. basilicum L. For industrial or antiseptic uses due to its richness in limonene, while the var. purple and citriodorum varieties can be directed towards medicinal and aromatic uses due to their content of linalool and pinene.
Keywords
HPLC; basil; Azotobacter; banana peels

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