The effect of physiological maturity on the content and stability of active compounds in chili pepper fruits (Capsicum frutescens L.) during storage

Muttar, Muna Salih; Ahmed, Taha Shihab; Ahmed, Mohamed Abdullah

doi:10.32894/MEDIP.26.4.1.4

	The effect of physiological maturity on the content and stability of active compounds in chili pepper fruits (Capsicum frutescens L.) during storage
Journal of Medicinal and Industrial Plant Sciences
Volume 4, Issue 1, April 2026, Pages 50-63 PDF (615.29 K)
Document Type: Original Article
DOI: 10.32894/MEDIP.26.4.1.4
Authors
Muna Salih Muttar^* ; Taha Shihab Ahmed; Mohamed Abdullah Ahmed
Department of Horticulture and Landscape, College of Agriculture, Tikrit University, Iraq.
Abstract
This study aimed to evaluate the impact of fruit maturity stage, storage container type, and storage duration on the content of active compounds and vitamins in hot pepper fruits (Capsicum frutescens L.), as these are important factors in maintaining the postharvest chemical quality and utility of food. The experiment was carried out in spring season of 2024 at the Horticultural Research Station, College of Agriculture, University Kirkuk, Its geographical coordinates are approximately (44.3) degrees east longitude and (35.3) degrees north latitude. according to Completely Randomized Design (CRD) and with three replication under three factors. The factors consisted of two maturity stages (green fruits A1 and red fruits A2), two storage container types (metallic B1 and clay B2) and three storage periods (45 days C1, 90 days C2, 135 days C3). Results were significant for all factors analysed. The maturity stage A2 outperformed in phenols, alkaloids and flavonoids while capsaicin and the vitamins studied were most abundant at stage A2. Successive extractions Efficiency of retaining active compounds was greater in clay (B2) than metal containers. In relation to storage period, 45 days was best for most of the traits and the highest capsaicin content a peel was observed at 135 days. The interactions demonstrated the dominance of A2B2C1 treatment phenols, alkaloids, vitamin A, B6 and C (280.1 mg 100g⁻¹, 1.1033 %, 123.67 ppm, 40.67 ppm, 199.77 ppm) respectively. The findings indicate that the late maturity and fermentation in clay pots, followed by short-term storage, help to decrease loss of oxidation-sensitive compounds because of the stabilization of storage conditions and minimization of metabolically mediated degradations. This validates the possibility of improving the chemical value of hot pepper fruits through proper postharvest handling. The findings indicate that the late maturity and fermentation in clay pots, followed by short-term storage, help to decrease loss of oxidation-sensitive
Keywords
Organic fertilizers; active ingredients; pinching; pepper plant

References
Al-Qaisi, M. R. M. (2020). Design and statistical analysis of biological experiments using GenStat software. College of Agriculture, Tikrit University (Forthcoming). Chanthal, S., Songsri, S., & Somboon, T. (2012). Determination of capsaicin and dihydrocapsaicin in Capsicum spp. by HPLC. International Food Research Journal, 19(3), 1099–1105. Davey, M. W., Van Montagu, M., Inzé, D., Sanmartin, M., Kanellis, A., Smirnoff, N., Benzie, I. J. J., Strain, J. J., Favell, D., & Fletcher, J. (2021). Plant L-ascorbic acid: Chemistry, function, metabolism, bioavailability and effects of processing. Journal of the Science of Food and Agriculture, 101(3), 1–12. https://doi.org/10.1002/jsfa.10829 Estrada, B., Pomar, F., Díaz, J., Merino, F., & Bernal, M. A. (2020). Capsaicinoids in vegetative organs of Capsicum annuum L. in relation to fruiting. Journal of Agricultural and Food Chemistry, 68(12), 3648–3656. https://doi.org/10.1021/acs.jafc.9b07542 Fikiru, T., Alemu, T., & Bekele, M. (2025). Effect of packaging materials on shelf life and bioactive compounds of chili pepper during storage. Heliyon, 11(1), e15789. https://doi.org/10.1016/j.heliyon.2024.e15789 Guijarro-Real, C., Prohens, J., Rodríguez-Burruezo, A., & Fita, A. (2023). Changes in capsaicinoids, phenolic compounds and antioxidant activity during fruit ripening in Capsicum species. Agronomy, 13(1), 165. https://doi.org/10.3390/agronomy13010165 Guijarro-Real, C., Prohens, J., Rodríguez-Burruezo, A., Fita, A., & López-Gresa, M. P. (2023). Changes in phenolic compounds and antioxidant capacity during fruit ripening of different Capsicum species. Antioxidants, 12(2), 215. https://doi.org/10.3390/antiox12020215 Hong, Y., Chen, J., & Liu, C. (2023). Impact of light, oxygen and storage conditions on oxidative degradation of bioactive compounds in vegetables. Postharvest Biology and Technology, 197, 112207. https://doi.org/10.1016/j.postharvbio.2023.112207 Howard, L. R., Talcott, S. T., Brenes, C. H., & Villalon, B. (2000). Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum species) as influenced by maturity. Journal of Agricultural and Food Chemistry, 48(5), 1713–1720. https://doi.org/10.1021/jf990916t Ibrahim, A. M., Hassan, M. S., & El-Sayed, S. A. (2024). Influence of storage container materials on quality attributes and phytochemical stability of chili pepper fruits. Journal of Food Quality, 2024, 1–10. https://doi.org/10.1155/2024/8894321 Iqbal, A., Khalil, I. A., Ateeq, N., & Khan, M. S. (2015). Nutritional quality and antioxidant properties of chili pepper as affected by storage duration. Journal of Food Science and Nutrition, 3(4), 321–329. Kim, S. Y., Jeong, S. M., Park, W. P., Nam, K. C., Ahn, D. U., & Lee, S. C. (2022). Effect of heating conditions on bioactive compounds of pepper (Capsicum annuum L.). Food Chemistry, 376, 131874. https://doi.org/10.1016/j.foodchem.2021.131874 Kovács, Z., Szőllősi, D., & Székely, G. (2022). Carotenoid accumulation and plastid transformation during fruit ripening of red pepper (Capsicum annuum L.). Plants, 11(3), 365. https://doi.org/10.3390/plants11030365 Lahbib, K., Dabbou, S., & Gazzah, M. (2023). Effect of storage conditions on bioactive compounds and antioxidant capacity of chili pepper fruits. Journal of Food Processing and Preservation, 47(2), e16854. https://doi.org/10.1111/jfpp.16854 Lahbib, K., Dabbou, S., Gazzah, M., & Ben Ahmed, S. (2023). Ripening stage effect on phytochemical composition and antioxidant activity of hot pepper (Capsicum frutescens L.). Scientia Horticulturae, 309, 111639. https://doi.org/10.1016/j.scienta.2022.111639 Manikharda, P., Xie, J., & Qian, J. (2018). Changes in capsaicinoid content and antioxidant activity of chili peppers during fruit development and ripening. Journal of Food Composition and Analysis, 67, 1–8. https://doi.org/10.1016/j.jfca.2017.12.008 Mozafar, A. (2019). Plant vitamins: Agronomic, physiological and nutritional aspects. CRC Press. Nisar, J., Khan, A., Ali, M., & Khan, M. R. (2021). Tocopherols and tocotrienols: Chemistry, sources, and health benefits. Journal of Food Biochemistry, 45(2), e13575. https://doi.org/10.1111/jfbc.13575 Payne, R., Murray, D., Harding, S., Baird, D., & Soutou, D. (2009). GenStat for Windows (12th ed.). VSN International. Ribes-Moya, A. M., Raigón, M. D., Moreno-Peris, E., Fita, A., & Rodríguez-Burruezo, A. (2020). Response to organic cultivation of hot pepper fruit quality. Scientia Horticulturae, 261, 108958. https://doi.org/10.1016/j.scienta.2019.108958 Rodriguez-Amaya, D. B. (2022). Update on natural food pigments—A mini-review on carotenoids, anthocyanins, and betalains. Food Research International, 155, 111002. https://doi.org/10.1016/j.foodres.2022.111002 Saini, R. K., Nile, S. H., & Park, S. W. (2021). Carotenoids from fruits and vegetables: Chemistry, analysis, occurrence, bioavailability and biological activities. Food Research International, 144, 110294. https://doi.org/10.1016/j.foodres.2021.110294 Santos, J. S., Escher, G. B., Ferreira, B. L., & Granato, D. (2021). The effect of storage time and temperature on bioactive compounds and antioxidant activity of fruits and vegetables. Food Chemistry, 336, 127657. https://doi.org/10.1016/j.foodchem.2020.127657 Sharaa, I. E., & Mussa, S. B. (2019). Determination of vitamin C (ascorbic acid) contents in vegetable samples by UV-spectrophotometry and redox titration methods. International Journal of Progressive Sciences and Technologies, 15(2), 281–293. Sora, G. T. S., Haminiuk, C. W. I., da Silva, M. V., Zielinski, A. A. F., & Gonçalves, G. A. (2021). A critical review of capsaicinoids: Occurrence, bioavailability, and health effects. Trends in Food Science & Technology, 112, 284–297. https://doi.org/10.1016/j.tifs.2021.04.018 Vázquez-Espinosa, M., González-de-Peredo, A. V., Espada-Bellido, E., Ferreiro-González, M., & Palma, M. (2020). Changes in capsaicinoids and phenolic compounds during the ripening of chili peppers. Food Chemistry, 312, 126051. https://doi.org/10.1016/j.foodchem.2019.126051 Versino, F., López, O. V., & García, M. A. (2023). Effect of packaging materials on oxygen permeability, moisture transfer and quality preservation of horticultural products. Food Packaging and Shelf Life, 35, 101010. https://doi.org/10.1016/j.fpsl.2023.101010 Villa-Rivera, M. G., Ochoa-Velasco, C. E., & Hernández-Carranza, P. (2023). Storage time and temperature effects on phenolics, vitamins and antioxidant capacity of chili pepper fruits. Food Chemistry, 405, 134858. Zhang, L., Li, S., Wang, A., & Ye, Y. (2022). Effects of packaging materials on the stability of phenolic compounds.
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