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In Silico Design and Analysis approach, Multi-Epitope Antigen Against Coccidiosis in Broiler Chickens | ||
| Al-Qadisiyah Journal of Veterinary Medicine Sciences | ||
| Volume 25, Issue 1, May 2026, Pages 203-215 PDF (1.01 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.29079/qjvms.2025.160830.1092 | ||
| Authors | ||
| haider hamza atshan* 1; Noor Idan Jarad2 | ||
| 1Veterinary Medicine College /Al-Qadisiyah University, Iraq | ||
| 2College of Veterinary Medicine, University of Al-Qadisiyah, Iraq, | ||
| Abstract | ||
| Background: The spread of coccidiosis in poultry has been accompanied by challenges in control and treatment, such as resistance to repeated treatments used in the field, and limited immunity due to antigenic diversity, which prompted researchers to adopt the concept of immunogenic antigens. Objectives: To generate a vaccine using computational methods to create broader immunity against mixed infections and longer-lasting immunity to control them. Methods: Using bioinformatics design techniques to provide the highest and most comprehensive level of protection against coccidiosis, the current project introduced the 19 kDa sporozoite antigen as an immunogenic protein genetically conserved among Eimeria species to design a multi-antigen vaccine. Bioinformatics tools were used to generate a vaccine antigen with a total of 18 antigens (B cells, cytotoxic T cells, and high-density T cells) consisting of 274 amino acids. Outcome: The reconstituted vaccine was physically and chemically evaluated, and binding to TLR15 and TLR4 was achieved. Dynamic immunological simulations were performed, using results from immunological compounds and molecules, including antigen levels of 700,000/ml and 500,000/ml, INFy about 4,75,000 ng/ml, and enhanced T and B cell proliferation to produce IgG (30,000/ml). Conclusion: The results of the vaccine design and evaluation simulations were acceptable expectations, and further in vivo experiments are needed to confirm the vaccine's defensive efficacy | ||
| Keywords | ||
| Eimeria; immunobioinformatic; antigen; vaccine; epitope | ||
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