Isolation and molecular study to free - living Naegleria gruberi from different source clinical and environmental in Karbala and Qadisiyah province / Iraq

Abbas, Zahraa Fadhil; Jasim, Ghaidaa Abass

doi:10.29079/qjvms.2025.150627.1019

	Isolation and molecular study to free - living Naegleria gruberi from different source clinical and environmental in Karbala and Qadisiyah province / Iraq
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 24, Special Issue ( 3 ) The 1st National and International Scientific Conference November 26 / 2025, January 2026, Pages 6-11 PDF (666.25 K)
Document Type: The 1st International Conference of Iraqi Colleges of Veterinary Medicine-المؤتمر الدولي الاول لكليات الطب البيطري العراقية
DOI: 10.29079/qjvms.2025.150627.1019
Authors
Zahraa Fadhil Abbas^* ¹; Ghaidaa Abass Jasim²
¹College of Applied Medical Sciences, University of Karbala, Karbala, Iraq.
²Department of Veterinary Microbiology and Parasitology, University of AL-Qadisiyah, Al-Diwaniyah City, Iraq
Abstract
This study was acted in Karbala and Qadisiyah governarat center of Iraq in perioed from Septemper to October 22 request to location of the crafty Free living amoebae (FLA) and recognized by morphological characters in culture, Polymeras chain response (PCR) by 18SrRNA general FLA primers. 181 samples were gathered from 123 clinical cases including, eye, skin, nose from creatures and human, and CSF from human and 58 samples were gathered from environmental samples were gathered from various sources of water , soil .All samples were refined in the Non Supplement agar medium (NN-agar medina) and then inspected by light microscope to perceived Morphologically the flagellate, trophozoite and cyst of opportunistic amoebas for 12/181 samples included 5/58(2.276%) environmental source (water and soil ) and 7/123( 3.867%) clinical(eye,skin,nose samples) from animals were positive to Naegleria spp. Ten from12 tests from environmental and clinical sources were disconnected of common FLA that were positive microscopically, the outcome was positive for three samples for Naegleria gruberi after analyzed sequences. The study of isolation and molecular diagnosis of Naegleria gruberi is the first of its kind in Iraq, and It is the first recorded in Iraq in the Gene Bank database.
Keywords
Naegleria gruberi; Parasite Epidemiology; Parasitic diseases

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