Optimal oral dose of VP24 dsRNA enhances survival and immunity in Penaeus monodon during WSSV challenge

Parenrengi, Andi; Suryati, Emma; Lante, Samuel; Rosmiati, Rosmiati; Aliah Hidayani, Andi; Sulaeman, Sulaeman; Herlinah, Herlinah; Pantjara, Brata; Subaidah, Siti; Alimuddin, Alimuddin

doi:10.33899/ijvs.2026.167243.4619

	Optimal oral dose of VP24 dsRNA enhances survival and immunity in Penaeus monodon during WSSV challenge
Iraqi Journal of Veterinary Sciences
Volume 40, Issue 2, April 2026, Pages 405-414 PDF (730.9 K)
Document Type: Research Paper
DOI: 10.33899/ijvs.2026.167243.4619
Authors
Andi Parenrengi^* ¹; Emma Suryati²; Samuel Lante²; Rosmiati Rosmiati²; Andi Aliah Hidayani³; Sulaeman Sulaeman²; Herlinah Herlinah⁴; Brata Pantjara⁵; Siti Subaidah²; Alimuddin Alimuddin⁶
¹Jalan Raya Jakarta-Bogor No.10. Cibinong, Kabupaten Bogor, Jawa Barat, 16912, Indonesia.
²Research Center for Marine Aquaculture, National Research and Innovation Agency (BRIN), Cibinong, Indonesia
³Fisheries Department, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar 90245, Indonesia
⁴Program Study of Technology in Aquaculture and Postharvest Fisheries, Faculty of Vocational Study, Universitas Hasanuddin, Makassar, Indonesia
⁵Research Center for Freshwater Aquaculture, National Research and Innovation Agency (BRIN), Cibinong, Indonesia
⁶Aquaculture Department, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, Indonesia
Abstract
The application of dsRNA was commonly conducted by injection and oral administration. Due to the limitations of muscular injection, delivering dsRNA by oral administration has been proven to be an effective approach for use in shrimp. One of the key factors in improving the effectiveness of the dsRNA vaccination is the concentration of dsRNA in the shrimp diet. This study was conducted to evaluate the influence of different dsRNA concentrations enriched in shrimp feed via oral vaccination on tiger shrimp. The dsRNA VP24 vaccine was produced by inactivating recombinant bacteria Escherichia coli DH5α using the heat-killed bacteria by the immersion technique. The density of inactivated bacteria (cells) was a reference to the dsRNA concentration in 0.02 g of shrimp feed, as the treatments, such as (A) control without dsRNA. (B) 107 cells, (C) 108 cells, and (D) 109 cells. The shrimp were challenged with WSSV by intramuscular injection with 50 µL/shrimp after 15 days of vaccination. The present study results showed that the different concentrations of dsRNA had a significant survival rate, proPO activity, and THC (P<0.05) in tiger shrimp. The highest survival rate (33.3%), proPO activity (0.054), and THC (5.39 × 106 cells/mL) were obtained in the concentration of 108 cells. The proPO gene also showed a higher expression in 108 cells than in the other treatments. This finding suggested that the optimal concentration of 108 cells per 0.02 g of shrimp feed was recommended to be applied for the VP24 dsRNA
Keywords
dsRNA concentration; immune response; survival; tiger shrimp; VP24 WSSV

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