- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Distribution and Prevalence of Aeromonas hydrophila Isolates from Clinical Cases 1 | ||
| Al-Qadisiyah Journal of Pure Science | ||
| Volume 31, Special, July 2026 PDF (923.58 K) | ||
| Document Type: IAAQC Conference | ||
| DOI: 10.29350/qjps.2026.168937.1017 | ||
| Authors | ||
| Ahmed Ali* 1; Azhar Hussein2 | ||
| 1Department of Biology, College of Education, University of Al-Qadisiyah, Iraq. | ||
| 2Department of Biology, College of Education, University of Al-Qadisiyah, Al-Diwaniyah, Iraq, | ||
| Abstract | ||
| The current research involved the investigation of the prevalence and laboratory patterns of Aeromonas hydrophila in human clinical samples. Two hundred clinical samples were examined of which 20 samples (10%) were positive to the A. hydrophila and 180 samples (90) were negative. The highest isolation rate was detected in stool samples of 15 positive isolates (15%), which showed gastrointestinal infections to have a strong association. The rates of lower isolation were found in urine samples (7%), and blood samples (6.7%). Wound/burn samples did not recover any isolates which implies that this pathogen does not play a major role in this type of infection among the population under study. The first classification was carried out on morphological basis. On both MacConkey agar and blood agar, all isolates grew well as non-lactose-fermenting colonies and gave smooth and hemolytic colonies respectively. The capability of growing in enriched media was indicated by the growth on chocolate agar. The microscopic observation revealed all the isolates to be Gram-negative bacteria. Anoxidase- and catalase-tests showed that all the isolates were positive, as expected of A. hydrophila. The system was identified by VITEK 2 Compact system with 100 percent consensus with the conventional methods. PCR showed the presence of the lipase gene in 90% and the protease gene in 100% of isolates, indicating the genetic presence of virulence-associated determinants. In general, the research confirms the clinical value of A. hydrophila, especially in gastrointestinal infections, and the importance of a combination of the conventional, automated, and molecular diagnostic methods. | ||
| Keywords | ||
| Aeromonas hydrophila; Clinical samples; Epidemiology; PCR; Virulence genes | ||
|
Statistics Article View: 42 PDF Download: 48 |
||