- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
A review on the effect of growth regulators on Stevia rebaudiana bertoni in vitro | ||
| Journal of Medicinal and Industrial Plant Sciences | ||
| Volume 4, Issue 2, June 2026, Pages 11-18 PDF (396.31 K) | ||
| Document Type: Article Review | ||
| DOI: 10.32894/MEDIP.26.4.2.2 | ||
| Authors | ||
| Zainab Hamid Abdul Rheem; ibrahim Ali Ameen* ; Nayyef Sabhan Khalaf | ||
| Department of Medicinal and Industrial Plants, College of Medicinal and Industrial Plants, University of Kirkuk, Kirkuk, Iraq. | ||
| Abstract | ||
| In recent years, there has been a marked increase in interest in the cultivation and utilization of medicinal plants for the extraction of therapeutic and pharmaceutical compounds as alternatives to chemically synthesized substances. Among these plants is Stevia rebaudiana, which belongs to the family Asteraceae. According to previous studies, the importance of Stevia also lies in its content of a wide range of antioxidant compounds, making it a valuable dietary supplement for cancer prevention. These compounds include quercetin, kaempferol, and other glycosidic compounds. This review highlights the importance of plant tissue culture technology in the propagation of medicinal plants, including Stevia rebaudiana, which is difficult to propagate through conventional methods due to the self-incompatibility of its seeds and their low fertility. Therefore, it has been successfully propagated via plant tissue culture techniques. The aim of this review is to highlight the necessity of employing plant tissue culture for the propagation of Stevia, as well as studying callus induction from different plant explants. In addition, it emphasizes the importance of using plant growth regulators at appropriate concentrations, as reported in the reviewed studies, to achieve optimal responses. | ||
| Keywords | ||
| Stevia rebaudiana; Plant tissue culture; Callus induction; Medicinal plants; Plant growth regulators | ||
| References | ||
|
Abdul Rahim, Z. H., & Jawad, L. K. (2021). The role of growth regulators in the multiplication of Stevia rebaudiana Bertoni shoots and callus induction in vitro. Diyala Agricultural Sciences Journal, 13(2), 24–31.
Abdul Razak, U. N. A. A., Ong, C. B., Yu, T. S., & Lau, L. K. (2014). In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia. Brazilian Archives of Biology and Technology, 57(1), 23–28. https://doi.org/10.1590/S1516-89132014000100004
Abdulhafiz, F., Mohammed, A., Reduan, M. F. H., Kari, Z. A., Wei, L. S., & Goh, K. W. (2022). Plant cell culture technologies: A promising alternative to produce high-value secondary metabolites. Arabian Journal of Chemistry, 15(11), 104161. https://doi.org/10.1016/j.arabjc.2022.104161
Aldabbagh, F. M. K., Al-Zaidi, I. H. M., & Alshamari, M. A. K. (2024). Micropropagation and assessment of genetic fidelity of regenerated plants using molecular markers. Iraqi Journal of Agricultural Sciences, 55(1), 432–439. https://doi.org/10.36103/tnx6m684
Al-Hasany, Z. H. A. R. (2021). The potential of increasing sweetening compounds and studying molecular markers in EMS-mutated tissue cultures of Stevia rebaudiana Bertoni (Doctoral dissertation, University of Baghdad).
Al-Sumaidaie, K. M. I. (2017). Applications in plant biotechnology (Part 1). Al-Nahrain University.
Anjali, S. (2021). Cytokinin and shoot development. Plant Science Today, 8(3), 123–130.
Attaya, A. S. (2021). Direct shoot bud proliferation protocol from Stevia rebaudiana leaf culture for healthy biomass production. Egyptian Journal of Agronomy, 43(1), 149–156.
Aziz, R. A., & Al-Taweel, S. (2019). Effect of plant growth retardants on Stevia rebaudiana Bertoni acclimatization produced in vitro. Plant Archives, 19(1), 1275–1284.
Cavallaro, V., Pellegrino, A., Muleo, R., & Forgione, I. (2022). Effects of light and plant growth regulators on in vitro proliferation. Plants, 11(7), 844. https://doi.org/10.3390/plants11070844
Deepak, S., & Tanveer, K. (2020). Plant tissue culture: Beyond being a tool for genetic engineering. In Environmental Microbiology and Biotechnology (pp. 175–200). Springer. https://doi.org/10.1007/978-981-15-6021-7_9
Eiji, N., & Saskia, C. M. V. (2021). Plant hormone functions and interactions in biological systems. The Plant Journal, 105(2), 287–289. https://doi.org/10.1111/tpj.1515
Goettemoeller, J., & Ching, A. (1999). Seed germination in Stevia rebaudiana. In Perspectives on New Crops and New Uses (pp. 510–511). ASHS Press.
Hussein, J. A. (2025). Tissue culture-based strategies for artificial seed technology in Stevia rebaudiana (Master’s thesis, University of Kirkuk).
Keshvari, T., Najaphy, A., Kahrizi, D., & Zebarjadi, A. (2018). Callus induction and somatic embryogenesis in Stevia rebaudiana Bertoni. Cellular and Molecular Biology, 64(2), 46–49.
Kudoyarova, G. (2022). Phytohormones. Biomolecules, 12, 1305. https://doi.org/10.3390/biom12091305
Kumar, A., Goswami, A., Sagar, A., Kumar, P., & Singh, R. (2019). Effect of plant growth regulators on in vitro callus induction and shoot proliferation of Stevia rebaudiana. Progressive Agriculture, 19(1), 118–122.
Kumari, P., Kumar, A., Priyadarshni, M., Kumari, R., & Shukla, L. N. (2017). Impact of different growth regulators supplemented in MS medium on callus induction from leaf explants of Stevia rebaudiana. International Journal of Research in Biosciences, 6(1), 14–18.
Lone, M. S., Hussain, K., Malik, A., Magray, M., Hussain, M. S., & Rashid, M. (2020). Plant propagation through tissue culture: A biotechnological intervention. International Journal of Current Microbiology and Applied Sciences, 9(5), 156–166.
Masri, M. I., Amein, M. M. M., Aziz, R. M. A., & Sayed, D. O. (2019). Callogenesis and plant regeneration via in vitro culture of Stevia rebaudiana explants. Egyptian Journal of Plant Breeding, 23(1), 65–76.
Mazzoni-Putman, S. M., Brumos, J., Zhao, C., Alonso, J. M., & Stepanova, A. N. (2021). Auxin interactions with other hormones in plant development. Cold Spring Harbor Perspectives in Biology, 13(10), a039990.
Neumann, K. H., Kumar, A., & Imani, J. (2020). Plant cell and tissue culture: A tool in biotechnology. Springer. https://doi.org/10.1007/978-3-030-49098-0
Obaid, L. M., & Abdulhalem, A. G. (2024). The influence of sucrose on biomass and glycosides content of callus cultured from Stevia rebaudiana. Iraqi Journal of Science, 65(7). https://doi.org/10.24996/ijs.2024.65.7.7
Sen, M. K., Nasrin, S., Rahman, S., & Jamal, A. H. (2014). In vitro callus induction and plantlet regeneration. Asian Pacific Journal of Tropical Biomedicine, 4(Suppl 1), S215–S219.
Sharma, N., Gauchan, D. P., Dhakal, A., Luitel, A., Shakya, S., & Shakya, R. (2015). Establishment of regenerative callus and molecular characterization of Stevia rebaudiana. IJRASET, 3, 133–144.
Singh, P., & Dwivedi, P. (2014). Two-stage culture procedure using thidiazuron for efficient micropropagation of Stevia rebaudiana. 3 Biotech, 4, 431–437. https://doi.org/10.1007/s13205-013-0169-9
Smith, R. H. (2000). Plant tissue culture: Techniques and experiments. Academic Press.
Sosnowski, J., Telesiński, A., & Jankowski, K. (2023). The impact of auxin and cytokinin on plant growth and development. Agriculture, 13(3), 724. https://doi.org/10.3390/agriculture13030724
Talal, N. R. N. (2023). The effect of growth regulators, light, and sucrose on micropropagation of Stevia rebaudiana (Master’s thesis, University of Kerbala).
Wawrosch, C., & Zotchev, S. B. (2021). Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook. Applied Microbiology and Biotechnology, 105(18), 6649–6668. https://doi.org/10.1007/s00253-021-11539-w | ||
|
Statistics Article View: 62 PDF Download: 58 |
||