Protective capability of nanoparticle soybean extract on Bali cattle spermatozoa during cryopreservation

Ratnawati, Dian; Irmawanti, Sulistiyoningtiyas; Nugraha, Chairdin Dwi; Hayati, Rini Nur; Anggraeny, Yenny Nur; Hayanti, Sari Yanti; Wardono, Heru Ponco; Rahayu, Sri; Ciptadi, Gatot; Susilawati, Trinil

doi:10.33899/ijvs.2026.168823.4693

	Protective capability of nanoparticle soybean extract on Bali cattle spermatozoa during cryopreservation
Iraqi Journal of Veterinary Sciences
Volume 40, Issue 3, July 2026, Pages 579-587 PDF (710.77 K)
Document Type: Research Paper
DOI: 10.33899/ijvs.2026.168823.4693
Authors
dian ratnawati^* ¹; Sulistiyoningtiyas Irmawanti²; Chairdin Dwi Nugraha²; Rini Nur Hayati²; Yenny Nur Anggraeny³; Sari Yanti Hayanti³; Heru Ponco Wardono²; Sri Rahayu⁴; Gatot Ciptadi⁵; Trinil Susilawati⁵
¹National Research and Innovation Agency
²Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency of The Republic of Indonesia (BRIN); Indonesia
³Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency of The Republic of Indonesia (BRIN)
⁴Department of Biology, Faculty of Mathematic and Natural Science, Universitas Brawijaya, Malang, Indonesia; Indonesia
⁵Department of Animal Reproduction and Breeding, Faculty of Animal Science, Universitas Brawijaya, Malang; Indonesia
Abstract
The purpose of this study was to examine formula diluents of CEP and nanoparticle soybean extract in maintaining the quality of frozen semen spermatozoa on Bali cattle. The research material used 1 Balinese cow. The research method was experimental with a Randomized Group Design, with 12 replicates (frozen semen). Treatment of frozen semen formula: T1 (CEP + KT 10%), T2 (CEP + EKN 50%), and T3 (CEP + EKN 60%). Parameters observed included: membrane integrity, acrosome integrity, motility, viability, abnormality, SOD, MDA, DNA Fragmentation and SEM. Data on membrane integrity, acrosome integrity, motility, viability, and abnormality were analyzed using ANOVA (SPSS 16). SOD, MDA, and Fragmentation DNA data were analyzed using Microsoft excel and SEM was presented descriptively. The results of the study of post thawing frozen semen showed progressive motility, viability of spermatozoa in group P1 was higher (p<0.05) than T2 and T3. The value of spermatozoa abnormalities and DNA fragmentation in the T1 group was lower (p<0.05) than T2 and T3. Diluent formula of CEP and 50% and 60% nanoparticle soybean extract can support the quality of frozen semen spermatozoa (viability, membrane integrity, acrosome integrity, and spermatozoa abnormality). Nevertheless the performance was still below CEP diluent and egg yolk. The motility characteristics are less progressive, slow movement speed, short travel distance, and low vigor. However, the swimming pattern was more linear, straighter, and does not tend to rotate. Post thawing progressive motility reached 51.35% and 45.07% and still feasible to use for artificial insemination.
Keywords
Bali cattle; freeze semen; nanoparticle; soybean extract

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