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Molecular identification of Entamoeba histolytica from patients infected with Helicobacter pylori | ||
| Journal of University of Anbar for Pure Science | ||
| Volume 20, Issue 2, August 2026, Pages 1-11 PDF (957.02 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2025.163132.1550 | ||
| Authors | ||
| Maysaa Najad Khataab* ; Reeda Nathem Hamoo | ||
| Department of Biology, College of Education for Girls, University of Mosul, Mosul, Iraq | ||
| Abstract | ||
| Infection with Entamoeba histolytica and Helicobacter pylori is considered among the most common and serious infectious diseases, particularly in developing countries. The current study aimed to evaluate the prevalence of E. histolytica among patients with H. pylori infection and gastrointestinal symptoms in Mosul city and to study the phylogenetic relationships of the isolated strains. A total of 200 tissue biopsies were collected from 100 individuals of both sexes who had abdominal pain accompanied by gastrointestinal symptoms, including 100 gastric biopsies and 100 duodenal biopsies, from August 1, 2024, to March 1, 2025. The samples were examined using histological examination and conventional polymerase chain reaction (PCR), respectively, to identify E. histolytica in patients with positive H. pylori infection. The current study showed that the prevalence of H. pylori infection was 76%. Furthermore, parasite identification test results showed a prevalence of 26.66% for E. histolytica. Genetic analysis of E. histolytica was conducted, and two new strains were isolated and registered at the National Center for Biotechnology Information (NCBI) under accession numbers PV151461 and PV132085. The phylogenetic tree analysis of the locally isolated E. histolytica strains, which were recorded in GenBank, demonstrated high similarity (100%) between the two strains identified in the current study. Strain PV151461 showed high similarity with Iraqi strains from Tikrit and Diwaniya, OL771810 and ON086988, respectively. Isolate PV132085 showed a significant match with the Iraqi isolates obtained from Tikrit. | ||
| Keywords | ||
| Entamoeba histolytica; Gastric biopsies; Helicobacter pylori | ||
| References | ||
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