Atiyah, R., AL-abady, F., Al-Helaly, L. (2026). Methionine adenosyltransferase (MAT) as a biomarker of oxidative stress in hemodialysis patients: assessment before and after dialysis and the effect of repeated treatment. , 20(2), 205-215. doi: 10.37652/juaps.2025.165981.1749
Rana Ibrahim Atiyah; Faiz M. AL-abady; Luay A. Al-Helaly. "Methionine adenosyltransferase (MAT) as a biomarker of oxidative stress in hemodialysis patients: assessment before and after dialysis and the effect of repeated treatment". , 20, 2, 2026, 205-215. doi: 10.37652/juaps.2025.165981.1749
Atiyah, R., AL-abady, F., Al-Helaly, L. (2026). 'Methionine adenosyltransferase (MAT) as a biomarker of oxidative stress in hemodialysis patients: assessment before and after dialysis and the effect of repeated treatment', , 20(2), pp. 205-215. doi: 10.37652/juaps.2025.165981.1749
Atiyah, R., AL-abady, F., Al-Helaly, L. Methionine adenosyltransferase (MAT) as a biomarker of oxidative stress in hemodialysis patients: assessment before and after dialysis and the effect of repeated treatment. , 2026; 20(2): 205-215. doi: 10.37652/juaps.2025.165981.1749

Methionine adenosyltransferase (MAT) as a biomarker of oxidative stress in hemodialysis patients: assessment before and after dialysis and the effect of repeated treatment

Journal of University of Anbar for Pure Science
Volume 20, Issue 2, August 2026, Pages 205-215    PDF (325.15 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2025.165981.1749
Authors
Rana Ibrahim Atiyah* 1; Faiz M. AL-abady2; Luay A. Al-Helaly3
1Department of Chemistry, College of Science, Tikrit University, Tikrit, Iraq
2Department of Chemistry, College of Science, University of Tikrit
3Department of Chemistry, College of Science, University of Mosul
Abstract
Chronic kidney disease (CKD) is commonly associated with elevated oxidative stress due to impaired renal clearance of reactive oxygen and nitrogen species. Hemodialysis (HD) may further exacerbate this imbalance. Methionine adenosyltransferase (MAT), a key enzyme involved in methylation and antioxidant pathways, may play an important role in redox regulation in patients with CKD. However, limited data are available regarding its behavior before and after HD and its association with oxidative and antioxidant markers. This study aimed to evaluate serum MAT levels in patients with CKD before and after HD and to assess their association with oxidative stress markers, including peroxynitrite (ONOO−)and malondialdehyde (MDA), and antioxidant markers, including glutathione (GSH), albumin, and uric acid (UA). This case-control study was conducted at Tikrit Teaching Hospital and Erbil Teaching Hospital. Serum samples were collected from 50 chronic HD patients before and after dialysis, in addition to 40 healthy controls. Serum levels of MAT, ONOO−, MDA, GSH, UA, and albumin were measured. Patients were further stratified according to dialysis frequency, including 12 versus 8 sessions per month. Statistical comparisons were performed using paired t-tests for pre- versus post-dialysis samples and independent t-tests for CKD patients versus controls and for the 12- versus 8-session groups. P values were reported for all comparisons. Compared with healthy controls, patients with CKD showed significantly higher mean levels of ONOO− (p = 0.006) and MDA (p = 0.001) and significantly lower levels of MAT, GSH, and albumin (p = 0.001). Uric acid levels did not differ significantly between the groups (p > 0.05). In paired comparisons of pre- and post-dialysis samples from the same patients, post-dialysis values were significantly higher for MAT (p = 0.038), ONOO− (p = 0.003), and albumin (p = 0.022), whereas GSH and UA were significantly lower after dialysis (p = 0.001). MDA showed a significant change after dialysis (p = 0.006). Compared with patients undergoing 8 dialysis sessions per month, those undergoing 12 sessions per month showed significantly lower levels of MAT ( p = 0.001), ONOO− (p = 0.010), and MDA (p = 0.032), whereas GSH was significantly higher (p = 0.019). No significant differences were observed in UA or albumin levels (p > 0.05). Oxidative stress was markedly elevated in patients with CKD and was not fully corrected by HD. Dialysis frequency appeared to influence MAT levels and redox balance. MAT may serve as a potential biomarker for monitoring antioxidant status and redox balance in patients with CKD undergoing HD.
Keywords
Antioxidants; Hemodialysis; Methionine Adenosyltransferase; Oxidative stress; Oxidants
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