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Influence of laser ablation parameters on the synthesis, characterization, and antibacterial activity of iron oxide nanoparticles | ||
| Journal of University of Anbar for Pure Science | ||
| Volume 20, Issue 2, August 2026, Pages 309-319 PDF (4.22 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2025.165745.1731 | ||
| Authors | ||
| Sahar Naji Rashid* 1; Awatif Sabir Jasim1; Kadhim A. Aadim2; Sumaya Zaidan Khalaf3 | ||
| 1Department of Physics, College of Science, University of Tikrit, Tikrit, Iraq | ||
| 2Department of Physics, College of Science, University of Baghdad, Baghdad, Iraq | ||
| 3Department of English, College of Arts, University of Tikrit, Tikrit, Iraq | ||
| Abstract | ||
| Pulsed laser ablation in liquid (PLAL) is a simple and rapid technique that enables the synthesis of nanoparticles (NPs) with high purity. It is a top-down physical approach based on the fragmentation of a bulk metal precursor. In this work, iron oxide nanoparticles (Fe3O4NPs) were synthesized using PLAL with an Nd laser at a pulse energy of 500 mJ . Two ablation wavelengths, 355 and 532 nm, and different pulse numbers, 500, 600, 700, 800, and 900 pulses, were used for both wavelengths to evaluate their effects on the optical properties of the synthesized NPs. Structural and morphological characterization was performed for the NPs synthesized using the highest number of pulses, 900 pulses, using several analytical techniques. The antibacterial activity of all synthesized NPs was then evaluated against four bacterial species isolated from the oral cavity: Streptococcus mutans, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The results showed that the synthesized NPs were spherical and exhibited a cubic polycrystalline phase, with average crystallite sizes of 17.47 and 20.39 nm for the samples synthesized at 355 and 532 nm , respectively. The lower ablation wavelength resulted in smaller average crystallite size, higher absorbance, and greater antibacterial activity compared with the longer wavelength. These findings indicate that Fe3O4 NPs synthesized by PLAL have antibacterial potential and may be considered for further investigation as supportive antimicrobial agents and for other biomedical applications. | ||
| Keywords | ||
| Antibacterial activity; Antibiotic alternative; Fe3O4 NPs; Laser ablation; Nano-colloidal | ||
| References | ||
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