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The antigenicity of Staphylococcus aureus in rats | ||
| JOURNAL OF THI-QAR SCIENCE | ||
| Article 1, Volume 2, Issue 1, 2011, Pages 11-19 | ||
| Authors | ||
| ; ; Shakir H. AL-Alwany; Alaa J. Hassan | ||
| Abstract | ||
| Staphylococcus aureus was isolated from patients of Hilla hospital for surgery .The killed antigen of S. aureus was prepared and mixed with incomplete Freund’s adjuvant then injected in rats . The results revealed that the effect of S. aureus antigen in cellular immunity which including ; phagocytosis ,endocytic killing by neutrophils and ingestion of bacteria by phagocytes at different times of incubation in rats primed with bacterial antigen . The means of Phagocytosis were reached 15.2، 35.4 ، and 50.7 after 10 ,30 and 50 min of incubation respectively ، while the means of endocytic killing were reached 4.8 ، 4.9 ، and 9.8 at the same times mentioned above . Furthere more ، the rates of ingestion were 0.48 ,0.178 and 0.196 at the same times respectively،while lower in above tests in control rats . The concentrations of total protein ، albumin and crude globulins were appeared lower in treated rats compared with control animals ، which reached 9.45 ,8.02 and 1.45 g/L, while it was 12.34 ,10.44 and 1.9 g/L for control group respectively .These results suggests that the killed antigen of S. aureus stimulated the cellular immunity ، while it was suppressive for humoral immunity in rats.Keyword :- Staphylococcus aureus. antigens . phagocytosis .Humoral . Immunity . Rats | ||
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