| A field experiment was conducted at summer season of 2005 according to split-split plot experiment using RCBD with three replicates. The purpose of the experiment was to study effects of mycorrhizal fungus, rock phosphate and potassium sulfate on some chemical properties of tobacco crop (Nicotiana tabacum ), Treatments included 2 mycorrhiza ( with, without) as main plots , phosphorus applied in three levels (0, 100 , 200 kg P ha -1) as subplots and potassium applied in three levels (0, 200 , 400 kg K ha -1) as sub-sub plots. Results showed that the application of potassium sulfate with mycorrhizal fungus caused a significant increase in height of the plant, which was 10.4 % when compared with non-inoculated plants. Application of mycorrhiza plus K (400 kg K ha –1) gave 90 cm plant height . Number of leaves was increased with mycorrhiza application giving 1.22 leaves compared with no mycorrhiza application and caused significant increase, by 7.9 %. The same trend of increase was seen of the surface area of leaves, by 14.8 % compared with non – inoculated plants. Weight of one m2 of dry leaves decreased from 72.76 g.m-2 for non mycorhizae treatment to 46.37 g.m-2 treatment with mycorhizael fungus. The percent of tobacco leaves ash increased with in creasing rates of P, K applied. Ash percent was 19.05 % with P2 treatment compared to P0 (16.67%) and 18.50% with K2 compared to K0 (17.22%) respectively. Mycorrhiza application increased % sugars in tobacco leaves by 17.70%, also P2 treatment was higher than P0 in 0.29% also potassium applied in K2 level increased the % of sugar by 0.09%. The three factors (mycorrhiza, phosphorus, potassium and their interactions) significantly decreased Cl concentration in tobacco leaves the treatment A1P2K2 gave 0.12% compared to control treatment (A0P0K0) gave 0.37%.The application of fungal bio fertilizer caused significantly increase in potassium concentration on tobacco dry leaves in a (20.4%) rate of increase as compared with non-inoculated treatment |