Effect of BA and TDZ in multiplication of shoot tips and nodes of Carnation in vitro

	Effect of BA and TDZ in multiplication of shoot tips and nodes of Carnation in vitro
Journal of Tikrit University For Agriculture Sciences
Article 1, Volume 11, Issue 1, 2011, Pages 126-135
Abstract
Shoot tips and nodes of two carnation cultivars "Jeanne Dionis Blano" with white flower and "Marie Chabaud Jaune" with yellow flower which taken from the field were cultured on MS medium supplemented with BA at (1.0 ,2.0 ,3.0) mg/l and TDZ at(0.1 ,0.5 ,1.0) mg/l, parts of leaf cultured on MS medium supplemented with 2,4-D at(0.5 ,1.0 ,2.0) mg/l for callus production, callus cultured on MS medium supplemented with NAA at( 0.1, 0.2,1.0) mg/l. Data refer, shoot tips responsibility were 100% from planting in vitro for both cultivars and different treatment , highest number of shoot tips 23.1 shoot/explant were obtained for planting shoot tips of yellow flower cv. on medium supplemented with 3.0 mg/l BA while planting shoot tips of white flower cv. on medium supplemented with 1.0 mg/l gave 21.0 shoot/explant ,highest number of shoot 30.0 shoot/explant were obtained form planting nodes of white flower cv. on medium supplemented 0.5 mg/l TDZ, callus initiation were 100% from parts of leaf cultured on MS medium supplemented with 2.0 mg/l 2,4-D, callus cultured on medium supplemented with 1.0 mg/l NAA gave 10.30 shoot/explant , plantlets produced in vitro acclimatized in laboratory, then transferred to greenhouse to grow naturally with survival 100%

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