| One-hundred eighty eight urine samples were collected from out patients with UTI attending Tikrit teaching hospital isolated bacteria were tested for their susceptibility of fourteen antibacterial agents. The prevalence of E. coli, P. aeruginosa, Pseudomonas sp., S. aureus, S. pyogenes were common accounting for 98(52%), 20(11%), 10(5%), 50(27%), 10(5%) , respectively. Among the tested antibiotics , the highest rates of resistance were reported in E. coli and P. aeruginosa, since they were resistant for most used antibiotics (claform, septrime, carbencillin, erythromycin, lincomycin, Spromycine, negram, gentamycin, carbencillin, ampicillin, cephalexin (keflex) and amoxicillin), also this study showed that 10 of 50 isolates of S. aureus were resistance to all used antibiotics in this study and 30(60%) of total isolates were susceptible to most antibiotics used in this study. While S, pyogenes Were resistance to all tested antibiotics. Since resistance plasmid have been increasingly associated in antibiotic resistance in both Gram positive and Gram negative bacteria infections we studied plasmid profile in E.coli and the results indicated two small plasmid found in E.coli. These findings demonstrate that resistance gene reservoirs are increasing in healthy persons |