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Extraction of Flavonoid compounds from Nigella Arvensis Linn seeds & to study their physiological effects on female reproductive system. | ||
| Medical Journal of Tikrit | ||
| Article 1, Volume 1, Issue 131, 2012, Pages 64-69 | ||
| Authors | ||
| Kahtan A. Al-Mzaein; Najdat Ali S. Al-Kadhi; Mossa M. Marbut | ||
| Abstract | ||
| Nigella arvensis is a grassy plant with green to blue flowers & small black seeds. It is known as seed of blessing belong to genus Nigella & family Ranculaceae. Black seeds have been used in public medicine as curative substances for treatment of many diseases. It has therapeutic uses like worm infestation treatment, antiallergic, antiviral & anti-inflammatory. Thus, the aim of this study is to extract, identify the flavonoid compounds from Nagella arvensis L. seeds (black seeds) & to study its physiological effective dose on female reproductive system of mature mice. The effect of different doses of extracted flavonoids on the ovarian and the uterine weight:- For assessment of effective physiological dose, daily different doses of the crude flavonoids (above or below than recommended dose 27.5 mg/kg of B.W. were given orally by gavage needle to 60 immature female mice, from weaning days (post natal day 17).Six groups of immature female mice including 10 mice/group were divided randomly into two subgroups for each. The first and the second subgroups were treated for one and two weeks respectively. The results of this study showed that each kilogram of Nigella arvensis dry seeds contained 1.211 gm of the flavonoid compounds. The high performance liquid chromatographic (HPLC) analysis indicated the existence of 12 flavonoids of which only seven were identified. The identified flavonoids included: Catechin, Merictin, Rutin, Hydroxy Quarcetin, Kaempherol, Quarcetin and Hesperdin at a level of 77.34, 52.3, 47.3, 396.0, 95.6, 267.5, 36.56 µg/ml of extract respectively. There is no significant differences in the ovarian and the uterine weights during the first week among the treated and the control groups were recorded. While, a significant increase was found during the second weeks in groups which received 40, 50 and 60 mg/kg B.W as compared to 20 and 30 mg/kg B.W. flavonoid treated and control groups. No significant differences among 40, 50 and 60 mg/kg B.W. flavonoid treated groups were observed. Hence, 50 mg of flavonoids/kg.B.W. was selected in this study as an effective physiological dose for the following experiments. | ||
| Keywords | ||
| Extraction of Flavonoid compounds from Nigella Arvensis Linn seeds; Arvensis | ||
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