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STUDYING OF THE OPTIMUM CONDITIONS TO PRODUCE INULINAS FROM A LOCAL ISOLATE OF Aspergillus niger J3 | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 39, Issue 5, 2008, Pages 52-60 | ||
| Author | ||
| Jasim M. Awda | ||
| Abstract | ||
| Four isolates of fungi grown on Jerusalem artichoke were subjected to screening process for select the highest producer one of the inulinase enzyme. The isolate J3 was found the highest producer. The isolate J3 was identified according as Aspergillus niger. Result showed that the optimum condition to produce the enzyme by solid state fermentation is by using dried Jerusalem artichoke as carbon source.For nitrogen source we used sodium nitrate, yeast extract, peptone and mixture of yeast extract with sodium nitrate in ratio 1:1 and 0.2% concentration. It has been found that the using mixture of yeast extract and sodium nitrate was given the highest activity of enzyme.Initial pH which was used is between (4-9) and it has been found that the optimum pH for producing of the enzyme was (5).The incubation temp was ranging from 20to 40C it has been found that 30C is the best temperature for incubation.Different incubation periods was taken ranging from 72hrs to 192 hr, and the optimum incubation period was found 168hr which given the highest activity of inulinase enzyme. | ||
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