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Enzyme lrnmtmoassay Technique for the Determination ofHormones inHumanSemm | ||
| Tikret Journal of Pharmaceutical Sciences | ||
| Article 1, Volume 4, Issue 2, 2008, Pages 152-164 | ||
| Authors | ||
| Ayub Alwaan Jassim; Hussian Hassan Kharnoob | ||
| Abstract | ||
| Fnz)•me Linked lmmunosassay. In the first step the hormone is ~aptured by a monoclonal antilx>dy bound to the wells of amicrometer plate. In the second step a biotinylated monoclonal antibody is added withstrcptavidin -peroxidase conjugate. 1 he biotinylated antibody hinds to the solid phase antibodycomplex and, in tum, binds the conjugntc. After incubation. the welb arc washed and theantigen complex lx>und to the well detected h) addition of chromogenic substrate. The intensity,,fthe coloration i~ proportional to the hormone concentration in the sample. ELISA technique lorthe quantitative determination of 'fn·iodothyroxine (T3), Tetra-iodothyroxine (I';). thyroidStimulating Jlormone (TSH). prolactin (Prol), Luteinizing hormone (Lll). Follicle StimulatingHom>one (FSH) and (Tcsto.) concentration was used. In present work the minimum detectableconcentrations MDC of hormones by this technique were estimated to be: | ||
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