| Aims: The aims of this 'tud~ "ere to ealuate the cO~ti>encss of olive lea•es extractmouthrinse on the dc•·cl"pment of plaque, gingivnls, periodontal pockets in differenrconcentrations as pure c.xtract 50%, apple cider vinegar (ACV) extract rinse 50%,ACV + olive leave extract (OLE) 15% in acidic medium, 20% ACV + OLEmouthrinsc compared with the ideal standard control mouthrinse chlorhexidine(CHX) 0.2%. Fifty adult volunteetS participated in thi~ controlled single blind crossOer 'tudy, 10 subj.:ch for each group, one control and four experimental groups »ithdifferent concentratiMs of the rinse for 1 minute twice daily during 8 weeks period, 2»t~lc~ in:er.al bcteen each 'isit for mothation and re>nforcement of application ofthe materiai.Thre.: applied indices (plaque and ginghal indices by USe and Silnes.}.CI'ITN index b} ·in:tmo rc~orded at baseline, after eaGh ••pcrimental period and atthe 1~1 visit, then c"'mining th( biological acti' it} of the material in the laboratory. Ithad been found that materials ere effective on the thn:e applied indices withsignificant percentage reduction (tor OLE 50%, ACV 50%) at p < 0.001 usingunpaired Z-test, and at p · 0.00 I, p < 0.0 I for OLI.: + ACV 5 t %, OLE + ACV 20%respective!} using unpaired Z-test except ror some visits for the two materials.Pc,-centage reduction, for the four applied concentrations were greatest for OJ E •ACV 20%, OLE- ·CV 15%. ACY. OLE respecti,ely for the three applied indice~with significant dilkrcnce' at p ~ 0.05 using repeated measureand Duncan t~. t,,ing OLI· mouthrinse in different acidic concentrations ofterbenetit in plaque. gingival. periodontal pocket depth n:ductions. but it is much lo"erthan that ofCHX 0.2"4 mouthrinse |