utilization of Lead citrate on the peripheral Nervous Systemin Rat (An Experimental and Histological Study)

Shahab Al-Tekrity, Sabah; Hameed Ibraheem, Ayad

	utilization of Lead citrate on the peripheral Nervous Systemin Rat (An Experimental and Histological Study)
Tikret Journal of Pharmaceutical Sciences
Article 1, Volume 6, Issue 1, 2010, Pages 10-16
Authors
sabah shahab Al-Tekrity; Ayad Hameed Ibraheem
Abstract
administrated of lead citrate solution with phosphate buffer saline orally in a dose of 250mg/kg for thirty days. Animais were divided into four groups. Group A survived for 30days. Group B for 60 days. Group C fbr 90 days and Groupb tzO days. Twenty animalswere administrated phosphate buffer saline for 30 clays and classified into four 4 groupsand survived for 30,60,90 and 120 days afterthe last dose. Atthe end of eachperiod theanimals were scarified and segments of the peripheral nerve (Sciatic nerve) wereremoved and fixed in osmic acid 1o/o , then processed in graded glycerin 500/o , pureglycerin for obtaining teased nerve fibers . Specimens tbr miiror"opi" examination weredone by application of the histological technique for obtaining transverse and longitudinalsections of the 6um thickness. The results of the present itr,iy d"*onstrated that theperipheral nerve fibers were affected after the 30,and 60 days mostly reflected by thepresence of the wallerian degeneration ( destrr-rction of the myelin sheath ancl axon) of themost nerve fibers and at the period of 90 days after the last dose was indicated aregeneration of the most nerve fibers , but still there cerlain percentage of the degenerateclnerve f-rbers and irregular outlines of its myelin sheath. After 120 days, Remyelination ofmost nerve fibers were detected associated with short internodes in between other intactnodes of Ranvier .

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