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Separation and determination of some water-solublevitamins in pharmaceutical preparation by IP RP-HPLC | ||
| Tikret Journal of Pharmaceutical Sciences | ||
| Article 1, Volume 1, Issue 1, 2005, Pages 15-29 | ||
| Authors | ||
| Retrwar O. Hassan; Sarmad B. Dikran; ; I. Faizullah | ||
| Abstract | ||
| A reversed phase(RP) HPLC with ion-pair reagent is used for simultaneousdeterminations of water-soluble vitarnins (nicotineamide, nicotinic acid, lolic acid,riboflavin (B2) and thiamin (B1)) in pharmaceutical preparations. The vitamins wereanalyzed using ZORBAX Cs (25cm x 4.6mm i.d) analytical column, 6-8pm particlesize, by isocratic elution with a mobile phase consisted of 20%methanol, bufferedwith 0.05M KH2PO4 (pH 3.5) , and 3x10-rM l-octanesulfonic acid as an ion-pairreagent at a flow rate of (1.Oml/min.). The UV-Visible detection of the separatedvitamins was made at 254nm for all water-soluble vitamins. The precision andaccuracy of method were checked by calculating RSD% and relative error E%, whichwhere found to be reasonable A RSD% between (0.5-4.76) 0% and relative errorlhetween (0.05-4.93)%.The limit of detection range was lound to be (0.0244,d'.0+:opglmt;. | ||
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