TREATING INFANT`S ROTAVIRUS DIARRHEA BY USING BABY FORMULA SUPPLEMENTED WITH IMMUNOGLOBULINS FROM BOVINE COLOSTRUM

Majeed H. Al- Samarraie, Abdul; A. Khafaji, Y.; A. Azeez, R.

	TREATING INFANT`S ROTAVIRUS DIARRHEA BY USING BABY FORMULA SUPPLEMENTED WITH IMMUNOGLOBULINS FROM BOVINE COLOSTRUM
The Iraqi Journal of Agricultural Science
Article 1, Volume 39, Issue 5, 2008, Pages 61-74
Authors
Abdul majeed H. Al- Samarraie; Y. A. Khafaji; R. A. Azeez
Abstract
Rotavirus was isolated from infant stools suffered from diarrhea. Pregnant cows were vaccinated subcutaneously by the isolated virus. From the colostrums of such cows immunoglobulin (lgs) were precipitated and then passed through Sephadex G-200 column to obtain pure IgG, IgA, IgM. The crude and purified Igs were tested through neutralization of virus in stools of 10 infected infants. It was shown that 1mg of crude antibodies when mixed with 1ml of stool could decrease the virus titer from 1:32- 1:256 to 0- 1:4 in 6 samples. When 1mg of every purified Igs fraction was mixed with 1ml of stool, it was shown that IgM, IgA and IgG reduced virus titer to zero in 10, 9and 7 samples, respectively. The rest of samples, either those treated with crude or purified Igs, showed titers between 1:2- 1:4. The crude Igs, after lyophilization, was used as a supplement in infant formula to treat 20 infants diagnosed with diarrhea. Ten of them received 750- 850 ml of milk supplemented with 1 g Igs/ l, The other ten received the same volume with 1.25 g Igs/ l . The diarrhea episodes were decreased within 48 hrs of the continuous treatment. The higher concentration at the end of treatment cured 60% of initial cases, compared to 20% cured in the lower concentration group, while the control group who received the unfortified milk continued at the same rate of diarrhea episodes.

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