THE EFFECT OF ELECTRIC SHOCK ON DNA STRUCTURE OF  Antirrhinum majus

D. Hassen, Kadhim; K. M. Ameen, Sami; K. Hussein, Janan

	THE EFFECT OF ELECTRIC SHOCK ON DNA STRUCTURE OF Antirrhinum majus
The Iraqi Journal of Agricultural Science
Article 1, Volume 39, Issue 5, 2008, Pages 38-51
Authors
Kadhim D. Hassen; Sami K. M. Ameen; Janan K. Hussein
Abstract
The experiment was conducted in the lathhouse of Horticulture Department – College of Agriculture – Baghdad University in fall season of 2006 to investigate the effect of electric shock (E.S.) on DNA of Antirrhinum majus.Three levels of E.S. severity AC (6,8,10 Ampere) and three timings of treating (2,4,6 minutes ) were tested plus the control. A special electric apparatus was prepared for this purpose. Sprouted seeds and seedlings were soaked (before the E.S. treatments) for 3h in a 1% NaCl solution. Then they soaked in a fresh water for the same period ( 3h ) before they planted in the soil. The genetic study included DNA finger printing for some selected plants of Antirrhinum majus. Testing Genetic Distance by using two DNA markers based on Polymerase Chain Reaction (PCR) was applied. Randomly Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) were employed. The study was carried out at International Center for Agricultural Research in the Dry Areas (ICARDA)-Aleppo-Syria. Antirrhinum majus showed clear morphological differences on either the vegetative or flowering growth. The genetic analyses by RAPD or AFLP and Genetic Distance for selected treated plants comparing to untreated (control) was performed. Highest Genetic Distance (42%) by RAPD markers and 27.5% by AFLP markers was recorded on plants that their sprouted seeds were treated by (8A X 6mins).

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