ARTIFICIAL SEED PRODUCTION BY ENCAPSULATING SOMATIC EMBRYOS AND SHOOT TIPS OF TWO TOMATO HYBRIDS

M.K.Al-Dabagh, Farqad

	ARTIFICIAL SEED PRODUCTION BY ENCAPSULATING SOMATIC EMBRYOS AND SHOOT TIPS OF TWO TOMATO HYBRIDS
The Iraqi Journal of Agricultural Science
Article 1, Volume 43, Issue 3, 2013, Pages 52-64
Author
Farqad M.K.Al-Dabagh
Abstract
Astudy was conducted at the tissue culture lab.College of Agriculture/University of Baghdad and Genetic Propagation Department and Biotechnology Department /Life Science College /Poznan University / Poland ,From January 2008 till April 2010,in order to produce synthetic seeds from somatic embryos and shoot tips and the effect of storage on percentage of seed conversion.With regard to the encapsulating somatic embryos and shoot tips,the results showed that the interaction of 2% SA (Sodium Alginate) and 12 mg/l CaCl2 gave the highest percentage of seed conversion (40%) for Choorouk,and the interaction of 2% SA with 8mg/l CaCl2 for shoot tips and the interaction of 2% SA with 12mg/l CaCl2 for somatic embryos and shoot tips,resulted in higher percentage of conversion rate,it was 40%.While this percentage increased to 90% when the encapsulating matrix supplied with 0.5mg/l BA (Benzyl adenine) and 0.05mg/l IBA (Indole butyric acid) for both hybrids. Conversion of encapsulated somatic embryos and shoot tips using alginate coating for both hybrids reached 90% following 14 days at 4c̊ and control treatment of storage A according to results of this study it can be concluded that to increase the percentage of seed conversion synthetic seed matrix should be enriched with plant growth regulators for both tomato hybrids .It is recommended to carry out further research and evaluation to test the encapsulation of nodes and epicotyls to examine the physiological roles of plant growth regulators such as GA3 and ABA supplied to synthetic seed matrix .

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