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Cytopathogenicity studied of fowl pox virus by using indirect immunoperoxidase and acridine orange tests | ||
| The Iraqi Journal of Veterinary Medicine (IJVM) | ||
| Article 2, Volume 29, Issue 2, 2005, Pages 12-20 PDF (0 K) | ||
| Authors | ||
| Suad Abdulkarim; Aied B. AL-Zughaibi; Harith M. AL-Hyali | ||
| Abstract | ||
| Locally isolated fowl pox virus was inoculated on chorioallantoic membrane of chick embryos, leads to the appearances of odema and pocks lesion on the membrane , 120 hours post inoculation .Virus assay showed that the infectivity titer were 106.8 to 107.5 EID50/0.1 ml in embryonated chicken eggs. Monolayer tissue cultures of chick embryo fibroblast cells infected with fowl pox virus were examined by acridine orange staining and indirect immunoperoxidase test to study the cytopathogenic effects of the virus . The most striking cytoplasmic changes observed was the presence of the intracytoplasmic inclusions at 24-48 hours post inoculation, numerous inclusions were clearly seen at 72 hr. P.I.in addition to cytoplasmic vaculation and granulation were clearly seen at 96 hr. P.I. These changes stained brilliant green with acridine orange and dark brown staining with immunoperoxidase . Both tests demonstrated the localization of pox virus antigens in infected cells at same intervals. | ||
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