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PURIFICATION OF GLUCOSE AND GALACTOSE SPECIFIC LECTINS FROM NIGELA SATIVA SEED | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 6, Issue 1, 2007, Pages 42-51 | ||
| Abstract | ||
| ABSTRACT Glucose specific lectin (GL) and galactose specific lectin (GaL) were purified from Nigela sativa at the first time in country. Purification steps included fractionation with ethanol (50-75%), Dialysis and affinity chromatography in sephadex (G-50) column for (GL) specific lectin and sepharose (4B) Column for (GaL) specific lectin. Lectin were purified to homogeneity as indicated by the presence of one band in polyacrylamid gel electrophoresis in the absence of denaturing conditions. The molecular weight of purified lectins measurements by gel filtration suggest values of 25000 and 50000 dalton for (GL) and (GaL) specific lectins respectively, and 25.000, 50.000 dalton by Polyacrylamide gel (SDS) electrophoresis. Keywords: Glucose specific lectin, galactose specific lectin affinity chromatography, polyacrylamid gel. PDF created | ||
| Keywords | ||
| Glucose specific lectin; galactose specific lectin affinity chromatography; polyacrylamid gel | ||
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