Antibiotic Resistance And Plasmid Contents Of Salmonella Serotypes Isolated From Raw Milk

K.Nazal, Khilud; H.Najim, Najim; A. Hassem, Mohammed

	Antibiotic Resistance And Plasmid Contents Of Salmonella Serotypes Isolated From Raw Milk
The Iraqi Journal of Veterinary Medicine (IJVM)
Article 1, Volume 31, Issue 1, 2007, Pages 28-40 PDF (0 K)
Authors
Khilud K.Nazal; Najim H.Najim; Mohammed A. Hassem
Abstract
This study lasted for eight months .The main objectives of the study were to identify the main sources of raw milk pollution with Salmonella with a special emphasis on determination of their serotypes. The relationship between their resistance to antibiotics and their plasmide contents were also studied. Six hundred raw milk samples collected randomly at weekly intervals where the first (300) samples were from the individual milk cans (50 Kg each) at the producers homes in Abu-Ghraib village while the second (300) samples were collected from the scale bulk tanks (2 tons) inside the milk reception and collection center at the same village. In addition to that (200) swabs were taken from four different location (50 swabs for each) namely: the teats, milkers hands, the inner surface of the milk cans and the sedimemts that were precipitated at the bottom of the scale bulk tank.Data revealed that 5% of the milk cans and 7% of the scale bulk tank were contaminated with Salmonella besides the percentage of the Salmonella isolates from different swabs location were as 10% , 30% , 8% and 52% out of the teats, inner surface of milk cans, milker’s hands and sediments of the bulk tanks respectively . Seven serotypes of Salmonella were identified from both milk samples and swabs including S. typhimurium 40 %, S. anatum 26.67%, S. Kottbus 13.33 %, S. infantis 6.67%, S. dublin6.67%, S. java 3.33% and S. typhi 3.33%. Antibiotic sensitivity test for different antibiotics revealed that most Salmonella isolates were resistant to two antibiotics where (36.66%)of the isolates were resistant to Sulphamethazol, (16.66%)were resistant to tetracycline , (10%) were resistant to Streptomycin and (3.33%) were resistant to each of Chloramphenicol , Kanamycin and Ampicillin. The extraction of the DNA-plsmide revealed that seven out of ten isolates contained large, individual plasmids size of (50-70) Killo Base (KB) while the others three isolate were free from such plamid.

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