Comparative cellular immune response study of anthraxin prepared by a modified method with antigens extracted from Bacillus anthracis

J.L. Al-Juboury, Am; S. Aziz, Zahid; M. Al-Khatib, Ghazi

	Comparative cellular immune response study of anthraxin prepared by a modified method with antigens extracted from Bacillus anthracis
The Iraqi Journal of Veterinary Medicine (IJVM)
Article 9, Volume 32, Issue 1, 2008, Pages 114-126 PDF (0 K)
Authors
am J.L. Al-Juboury; Zahid S. Aziz; Ghazi M. Al-Khatib
Abstract
The study was aimed to comparison of anthraxin prepared by a modified method from cell-wall extract of avirulent B .anthracis strain 34F2(Sterne) with antigens extracted from the same strain as 1/40 antigen, autoclaved 1/40 and the crude toxin. These antigens were compared on their induction of cell-mediated immunity (CMI) in guinea pigs. Animals were immunized and boosted subcutaneously with the Sterne live veterinary anthrax vaccine. Two weeks after the booster dose, animals were skin-tested with the four antigens. Anthraxin was the most active antigen which recorded 16.66 mm a mean of erythema and 2.3 mm a difference of skin thickness after 24 hours. Both 1/40 and autoclaved 1/40 antigens gave approximately the same results which were 12.5 mm as a mean of erythema and 2.25 mm skin thickness for the first one; and 12.8mm ,1.66mm for the other respectively. The toxin showed the lowest results of erythema 7.8 mm with edema. These antigens were also compared according to the histological changes on their sites of inoculation. Marked (typical) picture of delayed type of hypersensitivity (DTH) reaction was occurred for anthraxin.

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