Effect of mercury on the total and electrophoretic profile of proteins and on the activity of three enzymes in mantle of three species of freshwater clam : Unio tigridis,  Pseudontopsis euphraticus and Anodonta spp

Ali Taker, Abd; M. Ahmed, Sulaf

	Effect of mercury on the total and electrophoretic profile of proteins and on the activity of three enzymes in mantle of three species of freshwater clam : Unio tigridis, Pseudontopsis euphraticus and Anodonta spp
The Iraqi Journal of Veterinary Medicine (IJVM)
Article 13, Volume 32, Issue 1, 2008, Pages 166-179 PDF (0 K)
Authors
Abd Ali Taker; Sulaf M. Ahmed
Abstract
A three species of clam Unio tigridis, Pseudontopsis euphraticus and Anodonta sp. were collected from Al-Habaniya lake, Al-Angor region/Iraq .The animals were exposed to three concentrations of mercury :0.1, 0.2 and 0.4 mg/L . First group of animals were exposed for three weeks ,second group for two weeks and the third group for one week. At the end of the exposure period, the animals were dissected ,then the mantle removed for the further studies on proteins and enzymes. The results were : 1- Total protein concentration in the mantle decreased in the three species of clam exposed to mercury. It was observed that a prominent effect of mercury on the electrophoretic bands of proteins with the decrease in the intensity of protein bands or induction of new bands. 2- The activity of the three enzymes : Alkaline phosphatase , ALT and AST were not stable, either increasing or decreasing according to the different species and concentration of the mercury , for example the activity of alkaline phosphatase increased in the mantle of the first species at lower and decreased at the second concentration but decreased in third species at different concentrations . The activity of ALT increased in the mantle of the first species at all concentrations of mercuy, while the activity of AST decreased in the mantle of the first species and increased at the two other concentrations but decreased in the mantle of third species . 3- We concluded from this study that , the clam responses to the mercury in the environment, which caused a changes in proteins and enzymes. These changes can be used as an indicator to the water pollution with the mercury .

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