Effect of of Levamisole as immunostimulatoron the humoral and cellular immune response in sheep vaccinated with  Brucella melitensis Rev-1 vaccine

Yousif,; A.A,; A.K, Talal; Saigh, Al-

	Effect of of Levamisole as immunostimulatoron the humoral and cellular immune response in sheep vaccinated with Brucella melitensis Rev-1 vaccine
The Iraqi Journal of Veterinary Medicine (IJVM)
Article 12, Volume 32, Issue 2, 2008, Pages 120-139 PDF (0 K)
Authors
; ; Talal A.K; Al- saigh
Abstract
This study was designed to determined the influence of levamisole administration as immunostimulator for sheep vaccinated with Brucella melitensis vaccine (Rev-1). The sixty sheep (males and females) were divided equally into four groups The first group (vaccinated group ) the animals were vaccinated with Rev-1 vaccine at a dose of 1 ml s/c which contain 2 x 109 viable bacteria The second group ( vaccine and levamisole ), was vaccinated with Rev-1 as the same of the first group as well as levamisole was given orally at a dose of 10 mg / kg B.W a week before vaccination and with vaccination and a week after vaccination and then levamisole was given at monthly intervals up to the end of the experiment.* Third group (levamisole group) was given levamisole orally at a dose of 10 mg / kg B.W at the same regimen of the time of the second group. *The fourth group kept as a control group which was given 1 ml of PBS s/c The humeral and cellular immunity assessed on animals at 0 time ,2,4,6,8,10,12,14 weeks.The vaccinated animals showed a high temperature after vaccination in the Ist and 2nd groups, the highest rate value was recorded in the first group 40.93C. Same trends were noticed in respiratory and pulse rates. No changes were recorded in the third and fourth groups. The vaccine stimulate humeral immunity ( first and second groups ). Rose Bengal Test gave a positive result in both vaccinated groups only. The second group revealed high means significant antibody titer 1160.53 than the first group, while the third and fourth groups showed a negative result in the antibody titer as detected by passive haemagglutination test (PHA). While the cellular immunity showed the following results .Delayed type hypersensitivity test was positive in the vaccinated groups and the second groupshowed significantly higher average in redness area and its thickness, than the first group,while in third and fourth groups the results were negative . The E-Rosette test in second group showed significantly high activated and total lymphocytes ratio compared with the other groups followed the second,first group and fourth group didn’t show any increase than the normal ratio.. Serum total protein concentration was increased in the two groups which received the levamesol 2nd &3rd groups compared with 1st and 4th groups.

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