Comparative study between the prophylactics Effects of aqueous extract of Black Currant (Vitis vinferia.L) and vitamin E on some biological parameters related with heart diseases in oxidative Stressed rats

H, A.; Abdul-Katum,; Khudair,; K.K,

	Comparative study between the prophylactics Effects of aqueous extract of Black Currant (Vitis vinferia.L) and vitamin E on some biological parameters related with heart diseases in oxidative Stressed rats
The Iraqi Journal of Veterinary Medicine (IJVM)
Article 20, Volume 32, Issue 2, 2008, Pages 227-252 PDF (0 K)
Authors
A. H; ; ;
Abstract
This study was designed to investigate the prophylactic effect of aqueous extract of black currant concentrate on some biological markers related with heart disease in male rat treated H2O2. It also aimed at comparing the prophylactic effect of black currant concentrate to that of vit E. Forty adult male rats were divided randomly into equal groups (ten rat/group) & were treated as follows for 42 days. Rats in the first group (G І) were received normal water with oral intubation of sun flower oil 1ml /rat and consider as control group. Animals of the second group (G Π) were received 0.5% H2O2 in drinking water, while rats of the third group (G Ш) were received 0.5% H2O2 in drinking water with oral intubation of vit E 400 I.U/Kg.B.W diluting in sun flower oil for each rat daily .While animals in the fourth group (G ІV) were intubated daily 60mg/kg body weight of aqueous extract of black currant concentrate plus 0.5% H2O2 in drinking water. Fasting blood samples were collected at 0, 21, and 42 days of experiment to study the following parameters: A - Platelet count (PC) and prothrombin time (PT). B- Serum concentration of total cholesterol TC, triacylglycerol TAG, High density lipoprotein-cholesterol HDL-C, Low density lipoprotein-cholesterol LDL-C and Very low density lipoprotein- cholesterol VLDL-C of each group were measured. These parameters were regarded as biomarkers of atherosclerosis and coronary heart disease (CHD). Furthermore section of heart & aorta were assessed for histopathogical studies. The result revealed that administration of 0.5% H2O2 in drinking water for six weeks (42days) caused significant increase (p<0.05) in platelet count & in serum TC,TAG,LDL-C, and VLDL-C concentration with significant decrease(p<0.05) in prothrombin time and HDL-C concentration as compared to other groups, on other hand oral intubation of vitamin E or aqueous extract of black currant concentrate in addition to H2O2 (groups Ш and ІV respectively) decreased the serum concentration of TC, TAG, LDL-C, VLDL-C, and platelet count comparing to H2O2 treated group & the control. Besides, the black currant concentrate and vitamin E caused significant elevation in serum HDL-C concentration & prothrombin time. Histological study revealed that 0.5% H2O2 intubation initiated aortic atheromatus lesions characterized by collagen proliferation, thickness of the intima, infiltration of inflammatory cells& focal foamy cell in subintimal layer with narrowing of the blood vessels. While histological section of aorta& heart of H2O2 plus black currant concentrate showed complete regression of atheromatus lesions caused by H2O2 intubation. It seems that black currant concentrate exert protective actions against H2O2 induced oxidative stress, atherosclerosis & change in some biological Markers related to heart disease. Such effects were more effective than vitamin E in some parameters. According to the available literature it seems that this is the first study showed the antiatherosclerotic properties of aqueous extract of black currant concentrate & it’s more potent than vitamin E in this issue.

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