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THE APPLICATION OF SOME AGAR ALTERNATIVES IN MICROPROPAGATION OF THREE GRAPE(Vitis vinifera L.) VARIETIES | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 5, Issue 1, 2006, Pages 57-69 | ||
| Abstract | ||
| Some agar alternatives were used for solidifying the half strength MS medium using corn starch, potato starch, ice cream thickener (Thalabia) and a mixture of potato and gelatin. Those agents were tested for the micropropagation of three European grape varieties Vitis vinifera L. namely, Ollivate blane (Fransi Aswad), Halawani and Kamali. Results showed that using corn starch or potato starch instead of agar inconsistent in solidifying the culture media. Meanwhile, mixing potato starch with gelatin or ice cream thickner individually proved promising solidifying agents. The addition of 1 mg/ 1BA to the culture media increased the number and length of shoots significantly. No significant differences appeared between such gelling agent used. The three grape varieties produced roots characterized with its length and leaser branching when cultures on auxin free medium compared with those grown on auxin containing media. It is concluded that the three varieties of grape can be micropropagated on some cheap agar alternatives instead of the costly agar. ____________________________________________________________________ | ||
| Keywords | ||
| keywords; Grape; micropropagation | ||
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