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THE EFFECT OF PULSED NITROGEN LASER ON THE ELONGATION OF E.COLI MM294 AND pRP4 CONJUGABILITY | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 4, Issue 1, 2005, Pages 76-89 | ||
| Abstract | ||
| ABSTRACT This study analysed the effect of pulsed nitrogen laser: 337.1 nm wave length , 1.5 millijoule pulse energy,10 nanosecond pulse width and pulse repetition rate range from (1-50) pulse/ second on the viability of E.coli . Results indicated that the irradiation of E.coli MM294 containing pBR322 with repetition rate (8,16,32) pulse /sec for (1,5,10,25) min , respectively ,led to significant decrease in cell number proportional to the exposure time and number of pulses per second accompanied with elongation of some cells as expression for their sensitivity to UV laser radiation .The effect of N2- Laser on the conjugability of the resistance plasmid pULB113 was also studied . the plasmid carries resistant genes for Ampicillin , Tetracycline and Kanamycine sulphate. It also carries the transposon mini Mu ( pRP4::mini Mu). In this respect E.coli JMP39 containing pULB 113 was exposed to pulsed N2 laser for 20 min with pulse repetition rate of 30 pulse/ sec . The strain was then used as a donor in mating experiments with E.coli HB101 . The Millipore filters technique revealed that non irradiated transconjugants were grown on plates selective for the antibiotic markers with high frequency( 1.33 x 10ˉ³ ) but irradiated transconjugants were grown at much lower frequency (1x10¯5) which may indicates inactivation of the cells and mutation in the tra- genes. | ||
| Keywords | ||
| KEYWORDS; Plasmid; conjugation | ||
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