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APPROACH FOR PREPARATION OF RADIATION ATTENUATED EIMENIA TENELLA VACCINE IN BROILER AGAINST COCCIDIOSIS | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 4, Issue 1, 2005, Pages 16-29 | ||
| Abstract | ||
| ABSTRACT The aim of this research was to standardize the locally prepared vaccine against coccidiosis . The mature oocysts of Eimenia tenella were attenuated by gamma – radiation (200 gray) that did not affect sporozoits excystation . A one – day old male broilers were used which vaccinated by oral administration with two different doses (5×10 3 and 3×103 oocyst / chick ) at 14th day old .The clinical signs , mortalities , weight gains, pathological lesions, packed cell volume and number of oocyst in cecal content were examined . In order to examine the efficacy of vaccine to produce a protective immunity, the vaccinated groups were challenged on the 20th day post vaccination , with virulent E. tenella strain at dose of (5×104 oocysts) . This experiment demonstrated high levels of protection (99%) against the disease specially with 3×103 oocysts /chick . This vaccination program gave good weight gains without any clinical signs till marketing compared with infected group | ||
| Keywords | ||
| Vaccine; Eimenia tenella; broiler | ||
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