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THE PHYSIOLOGICAL EFFECT OF GONADOTROPINS FOR INDUCTION OF SUPEROVULATION AND IN VITRO OOCYTE MATURATION AND FERTILIZATION IN MICE | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 4, Issue 1, 2005, Pages 30-50 | ||
| Abstract | ||
| ABSTRACT It was considered that the selection of appropriate superovulation program and in vitro maturation (IVM) by using culture media has important steps for successful of assisted reproductive techniques for medical or economical or research purposes. Therefore, this study was designed to: 1- Determination the best superovulation program in mice, and 2- Determination the effect of using of culture media on IVM and in vitro fertilization (IVF) and embryonic development later in mice. Four hundred and twenty females were divided into seven groups and injected with (10 or 15 or 30) IU dose of either pregnant mare serum gonadotropin (PMSG) or human menopausal gonadotropin (hMG), in addition to control group was injected with 0.2 ml of normal saline. Also, natural mating was achieved for fifty superovulated and fifty non superovulated females. Furthermore, nine hundred and ninety five oocytes were incubated within TCM-199 and RPMI-1640 for IVM. Then, matured oocytes and active spermatozoa were incubated withinMEM for IVF. Results of superovulation programs, number of births, percentages of IVM and IVF were registered. Results of the present study appeared that the injection of PMSG has results better than the hMG. Also, injection of dose 10 IU has results better than the doses 15 IU and 30 IU. Non significant differences in the number of births between superovulated and non superovulated females were reported. Also, insignificant differences in IVM between TCM-199 and RPMI-1640 were noticed. It was observed that the MEM has good ability for IVF. From the results of this study, it was concluded that the injectionof 10 IU of PMSGfor female mouse was effective to induce superovulaton. Also, the importance to use culture medium MEM for IVF of oocytes matured in vitro using culture media TCM-199 and RPMI-1640. | ||
| Keywords | ||
| fertilization; Superovulation Program; Gonadotropins | ||
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