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PRUIFICATION OF DNASE PRODUCED BY LOCAL STREPTOMYCES ISOLATES | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 4, Issue 1, 2005, Pages 90-107 | ||
| Abstract | ||
| ABSTRACT The DNase enzyme which produced from local (Streptomyces sp.SH-10) was purified by fractionation by aqueous two phase system followed gel filtration through Sephacryl S-200 by two steps. The final purification folds was 4.33and the yield of the enzyme was 28.8% . The molecular weight of the purified enzyme determined by gel filtration was 19950, The optimum pH for enzyme activity and stability were 7.0 and 6.5-7.5 respectively, and the optimum temperature for enzyme activity was 35oC . ___________________________________________________ | ||
| Keywords | ||
| DNase; Purification; Streptomyces; Two phase phase | ||
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