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THE GENETIC VARIATIONS INDUCED IN Mathiola incana BY ELECTRIC SHOCK AS TESTED BY RAPD | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 40, Issue 5, 2009, Pages 111-123 | ||
| Authors | ||
| Kadhim D. Hassen al-jebory; Sami K. M. Ameen; Janan K. Hussein | ||
| Abstract | ||
| The study was conducted in the lathhouse of Horticulture Department – College of Agriculture – Baghdad University in fall season 2005 to investigate the effect of electric shock on DNA of Mathiola incana.Three levels of electric current severity AC (6,8,10 Ampere) and three timings of electric shock (2,4,6 minutes ) were tested plus the control treatment. A special electric apparatus was prepared for this purpose. Sprouted seeds and seedlings were soaked before the treatments for 3hrs in a 1% NaCl solution. Then they soaked in a fresh water for the same period ( 3hrs ) before they planted in the soil. The genetic study included DNA finger printing for some selected plants of Mathiola incana. The Genetic Distance by using Randomly Amplified Polymorphic DNA (RAPD based on Polymerase Chain Reaction (PCR) were applied. The study was carried out at International Center for Agricultural Research in the Dry Areas (ICARDA)-Aleppo-Syria. Mathiola incana showed clear morphological differences on either the vegetative or flowering growth. The genetic analyses by using RAPD and Genetic Distance for selected treated plants comparing to untreated plants(control) was performed. High Genetic Distance of Mathiola incana plants(35%) in RAPD markers was registered on plants which the sprouted seeds were treated by (10A X 4mins). | ||
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