- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Extraction, Identification and Antibacterial activity of Cyperus oil from Iraqi C. rotundus | ||
| Engineering and Technology Journal | ||
| Article 1, Volume 26, Issue 10, 2008, Pages 1156-1163 PDF (181.18 K) | ||
| DOI: 10.30684/etj.26.10.1 | ||
| Authors | ||
| Zeid abdul-Majid Nima; Majid Sakhi Jabier; Raghidah Ismaeel Wagi; Huda Abd Al-Kareem Hussain | ||
| Abstract | ||
| Cyperus rotundus has many different uses and these were based on the different parts of plant. The medical uses of cyperus have been used in medicine for thousands of years. The parts of the cyperus used are its leaves, seeds and oil. The Extraction process was carried out by steam distillation. Optimum organic extractant determined. The collected oil was identified via Thin Layer Chromatography (TLC) using a mixture of Ethylacetate: toluene (1:9) as chromatographic eluent . This study was designed to extract and identify of essential cyperus oil from C. rotundus .The Antibacterial activity of Cyperus oil was studied for various microorganisms (Staphylococcus aureus , Klebsiella pneumoniae, Proteus vulgaris, Streptococcus pyogenes , Eschirichia coli and Pseudomonas aeruginosa ) using inhibition zone method (Aromatogram). The MIC and MBC for each microbe were estimated. The oil of C. rotundus was shown a remarkable activity against gram-positive bacteria, less antibacterial activity was found against gram–negative bacteria and no activity was observed with the oil against Pseudomonas aeruginosa and Proteus vulgaris. Novel method for extraction and identification of chemical composition for Iraqi cyperus oil was conducted. The study of the biological activities of this oil is very important because of needing to be determined whether there is any correlation between the biological activities and one or more of the chemical compounds purified from C. rotundus oil. | ||
| Keywords | ||
| cyperus oil; antibacterial activity; Thin layer chromatography | ||
|
Statistics Article View: 291 PDF Download: 177 |
||