Two isolated bacterial strains (E. coli 64, E. coli MM294), were purified, they are: gram-negative bacteria, circular, regular edged, thick somewhat glitter, viscous, and lactose fermenter. They belong to the family of Enterobacteriaceae E. coli 64 showed the resistant to all used antibiotics except Rifampicin (RA), Cephotaxime (CTX), Nitrofurantion (FT), Tabramycin (TM), and Cephalexin (RX) (Sensitive to). While E. coli MM294 showed the sensitivity to all used antibiotics except: Rifampicin (RA), Lincomycin (L), Clindamycin (CM), and Erythromycin (E), (resistantt to) Conjugative experiments showed that E. coli strain 64 harbored a self transmissible plasmid carrying resistant genes for: Tetracycline (TE), Amoxicillin (AMX), Neomycin (N), Chloramphenicol (C), Ampicillin (AM), and Trimethoprime + Sulfamethorazole (SXT) It seems that all these resistant genes may be carried on one self transmissible plasmid, or they may be carried on one self transmissible plasmid (except Neomycin, resistant gene may carry on other self transmissible plasmid).. |