ANTIMUTAGENIC EFFECT OF ROCKET (Eruca sativa ) ON INDUCTION OF STREPTOMYCIN AND RIFAMPICIN RESISTANT MUTANTS IN BACTERIAL SYSTEMS

A. Khalaf, Ilham

doi:10.33899/magrj.2008.27317

	ANTIMUTAGENIC EFFECT OF ROCKET (Eruca sativa ) ON INDUCTION OF STREPTOMYCIN AND RIFAMPICIN RESISTANT MUTANTS IN BACTERIAL SYSTEMS
Mesopotamia Journal of Agriculture
Article 16, Volume 36, Issue 4, 2008, Pages 151-161 PDF (0 K)
DOI: 10.33899/magrj.2008.27317
Author
Ilham A. Khalaf
Abstract
G- system (Bacterial system) , composed of three strains , G3 (Bacillus spp) , G12 (Artherobacter spp) , G27 (Brevibacterium spp) which are Gram positive , sensitive to crystal violet and Streptomycin (10 µg / ml) and Rifampicin (20µg / ml) (Chromosomal markers) . This system was used to study the mutagenic effect of rocket juice (Eruca sativa) and its antimutagenic activity against the cyclophosphamide(Cp) mutagenic activity using different combinations by measuring the cytotoxicity (Estimating the survival index) , and genotoxicity by estimating the induction of streptomycin and rifampicin resistant mutants (As chromosomal markers) . Results showed that rocket juice at 50 and 100 µl / ml of log culture cell suspension in phosphate buffer had no cytotoxic or genotoxic effects , higher levels (200µg / ml) had slight cytotoxic and genotoxic activities . Cp had both cytotoxic and genotoxic activities . Combinations of plant juice and mutagen (Cp) together (J+Cp) or using juice before treating with mutagen (J/Cp) or using the juice after treatment with mutagen (Cp/J) showed different effects . The treatment (J+Cp) and (J/Cp)protect the cells and the survival index didn't exceed the cutoff value of mutagenic agents , while the (Cp/J) treatment was unable to protect the cells . The latter effects appeared as an induction of streptomycin and rifampicin resistance .

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