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ISOLATION, IDENTIFICATION AND PARTIAL PURIFICATION OF DIHYDROFOLATE SYNTHETASE FROM HYPOTYL STEMS CALLUS OF LETTUSE PLANT ( Lactuca sativa L. ) | ||
| Mesopotamia Journal of Agriculture | ||
| Article 17, Volume 36, Issue 4, 2008, Pages 162-169 PDF (0 K) | ||
| DOI: 10.33899/magrj.2008.27334 | ||
| Author | ||
| Sajida A-Abood | ||
| Abstract | ||
| Callus from hypocotyl explants of lettuce ( Lactuca sativa L.) was initiated on a modified Murashige and Skoog medium containing 1.0 mg/l of benzyladenine (BA) and naphthaleneacetic acid ( NAA ) .Dihydrofolate synthetase (EC.6.3.2.12) was found in lettuce callus and purified about 36 fold by treating with ethanol and chloroform, ammonium sulfate and gel filtration on Sephadex G-200. The molecular weight of dihydrofolate synthetase was found to be around 40 KD. By Sephadex G-200 column chromatography. The optimum pH for the activity was 6.8 . The optimum reaction temperature was 32C°. The Michaelis constants using dihydropetroic acid and adenosinetriphosphate were 0.036x10-3M and 5.263x10-3M respectively. | ||
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