EFFECT OF OVER DOSE SYNTHETIC ESTRADIAL 17- β HORMONE ON SOME PERIPHERAL BLOOD PARAMETERS AND BONE MARROW STEM CELLS IN ADULT FEMALE RATS

Jaffar Mohamad Ali Pedawy, Araz

doi:10.33899/magrj.2009.27495

	EFFECT OF OVER DOSE SYNTHETIC ESTRADIAL 17- β HORMONE ON SOME PERIPHERAL BLOOD PARAMETERS AND BONE MARROW STEM CELLS IN ADULT FEMALE RATS
Mesopotamia Journal of Agriculture
Article 1, Volume 37, Issue 4, 2009, Pages 2-8 PDF (0 K)
DOI: 10.33899/magrj.2009.27495
Author
Araz Jaffar Mohamad Ali Pedawy
Abstract
This research was conducted on Animal Production Department's laboratories / University of Duhok, from the beginning of July to the end of October 2006, by using (36) female rats to investigate the effect of over dose of gonad hormone (estradiol 17-β) with dose of 0.5 mg injected intraperitonealy twice a day for 1 week. The rats were divided into three groups (12 rats for each) the first group was the control and the second injected with estradiol, while the third group was as the second one but the measurement of studied parameters were taken after 7 days after hormone injection. Some hematological parameters from blood samples and stem's cells of bone marrow were studied at 12 weeks of rats age. The results showed no significant differences effect in the (RBC) count , (Hb), Basophiles percent and Band Basophiles among the three experimental groups, while significant (P≤0.05) depression in PCV among the control and the third group , second group, respectively. Significant (P≤0.05) increase were observed in WBC between the control and the second group , third group and significance (P≤0.05) increase in the Neutrophiles and Monocytes between the control and the third group and the second and the third group, respectively, as compared with significant (P≤0.05) decrease in the Lymphocytes between the control and the third group and the two treated groups. However, significant difference (P≤0.05) decrease in Early erythrocytes were noticed between control and the second group and significant (P≤0.05) increase between the control and the third group and the two treated groups respectively, while significant (P≤0.05) increase were noticed in Intermediate erythrocytes and Late erythrocytes and Band neutrophiles between the three groups respectively. Also significant (P≤0.05) increase in Eosinophiles % and Band eosinophiles were noticed between the control and the second group , third group, and significant (P≤0.05) increase in Myelocytes number and Megacaryocytes were noticed between the second and the third group only. We concluded that the estradiol 17- β treatment neither suppress erthropoisis nor increase the immune system cells, but it caused a lymphoma in the peripheral blood.

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