- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
ESTABLISHMENT OF REPRODUCIBLE LYMPHOBLASTOID CELL LINES | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 4, Issue 1, 2005, Pages 3-9 | ||
| Authors | ||
| Nidal Abdul-Muhymen; Layla Al-Omar; Ismail I. Latif | ||
| Abstract | ||
| Background: A whole blood culture started with mononuclear cell fraction would contain several different cell types. Nevertheless, the T-lymphocytes can be cultured specifically and will out grow the others, eventually resulting in highly enriched population. Objective: The study was conducted as a trial of establishment of lymphocytes cell lines. This series of experiments were done for five selected subjects. The same protocols were applied to all individuals. Method: One ml of heparinized blood (HB) was cultured in tissue culture flask containing 9 ml stimulation medium. The flask was incubated at 37ºC in CO2 incubator for 3 days. Then every three days 1ml of whole blood culture from the flasks was subcultured into other tissue culture flask containing 9 ml stimulation medium, until visible suspension of mononuclear cells intervening agglutinated red blood cells were seen within two weeks of culture. Results: After the whole blood mononuclear cells cultures were maintained up to two weeks, the mononuclear cells were separated. Twenty four-hour incubation of these separated lymphocytes in stimulation medium, pure rich mononuclear cells were obtained and seen under inverted microscope. Conclusion: A new method for cultivation of lymphocytes from whole blood was developed for the first time and probably no other reported comparable method conducted elsewhere until know. Pure lymphocytes cultures were established, and maintained cell division. The potentiality of human peripheral lymphocytes themselves to act as precursor has been demonstrated in vitro by the cloning of established lymphoblastoid cell line. Different procedures for generation of T- cell lines were outlined. No feeder cells were needed in our protocol or the addition of IL2. Key words: lymphocyte culture, cell line, Phytohemagglutinin | ||
| Keywords | ||
| lymphocyte culture; cell line; Phytohemagglutinin | ||
|
Statistics Article View: 121 PDF Download: 36 |
||