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Preparation of ELISA Kit for detection of Brucella infection | ||
| Engineering and Technology Journal | ||
| Article 1, Volume 28, Issue 13, 2010, Pages 618-626 PDF (188.79 K) | ||
| DOI: 10.30684/etj.28.13.14 | ||
| Authors | ||
| ابراهيم عبدالحسين الزبيدي; اسيل محمد حمزه; جنان محمود خلف | ||
| Abstract | ||
| Preparation of antigen from Br.melitensis strain Rev1 was done by using ultrasonication, Column chromatography used to analysis of protein component of antigen; four peaks on chromatography were observed. Electrophoresis techniques used for characterization of antigen, different in molecular weight (M.W) protein bands were showed in electrophoresis ranged between 478630-123060 Dalton. A group of guinea pigs was infected experimentally with virulent strain of Brucella abortus & Brucella melitensis.Peak that obtained by chromatography were used to indicate Brucella infection with the 2 virulent strains (Brucella abortus & Brucella melitensis). The best result obtained by using peak two which have the protein concentration 364.2 μg/ml a comparative study was done using a kit prepared by Synbiotics Company and the result was positive in Brucella abortus infection and negative in Brucella melitensis infection. | ||
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