CELLULASES PRODUCTION FROM LOCAL ASPERGILL US SP. ISOLATE AND ASSESING SOME OF THEIR PROPERTIES AND APPLICATIONS4- STUDYING SOME PROPERTIES OF CELLULASE AND CELLUBIASE

Th. Alravvi, Akram; H.A. Alani, Aswan

	CELLULASES PRODUCTION FROM LOCAL ASPERGILL US SP. ISOLATE AND ASSESING SOME OF THEIR PROPERTIES AND APPLICATIONS4- STUDYING SOME PROPERTIES OF CELLULASE AND CELLUBIASE
The Iraqi Journal of Agricultural Science
Article 1, Volume 38, Issue 5, 2007, Pages 34-54
Authors
Akram Th. Alravvi; Aswan H.A. Alani
Abstract
Certain properties of two enzymes cellulase and cellubiase which produced by Aspergillus sp. A18 were investigated. The optimum pH were 5.0 and 7.0, respectively, pH stability were between 5.0-7.0 for both. The enzyme (BC1) retained 100% of its original activity after incubation at 40°C for 60 min., while 30% of the original activity was lost at 50°C for 60 min. The enzyme (BCb2) retained 100% of its original activity after incubation at 60°C for 60 min. The optimum temp, for (BC1) was 40°C while it was 50°C for (BCb2).The values of Km for BC1 were estimated by using three kinds of substrates, cellulose, treated CMC and treated wheat straw, were 4,0.8 and 0.33 mg respectively. Vmax values were 41.66,20 and 10 u mole/hr., respectively. For BCb2, Km and Vmax values were estimated by using two substrates which were cellobiose and treated CMC. Km values were 7.69 and 0.28 mg, respectively, while Vmax values were 57.14 and 16.66 p mole/hr., respectively. Isoelectric point for BC1 was found to be at pH 3.6 and for BCb2 at pH 4.2. Molecular weight determinations for BCI and BCb2 by gel filtration were 104 and 95 KD respectively, while they were 81.28 and 72.44 KD, respectively as determined by SDS- electrophoresis. It was found that BCI without any carbohydrate while BCb2 consisted of 13.7% carbohydrates. The main final reaction products as analyzed by (PC) and (TLC) were shown to be glucose and cellobiose.

Statistics Article View: 116 PDF Download: 21